ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI FROM RETAIL MARKET MUTTON
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Date
2004
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AAU, Anand
Abstract
This study was undertaken in order to isolate and identify Escherichia coli (E.coli) from different retail market mutton samples as well as from butchers' hand and their instruments for study of their cultural characteristics and to judge the hygienic level of different mutton shops of local market. A total of 150 samples were collected comprising of liver, lymph node, muscle, swab of hand, knife and log (25 samples each) from five different retail mutton shops of Anand, Gujarat. During the study 100 (66.66%) E.coli isolates were recovered using MacConkey agar and Eosin Methylene blue agar as different and selective plating media. Highest prevalence of E.coli was recovered from muscle (88.00% followed by liver (84.00%), lymph node (68.00%), knife swab (60.00%), log swab (52.00%) and least from butchers' hand (48.00%). All recovered 100 E.coli isolates were studied for biotyping, serotyping, in vitro antimicrobial sensitivity, Congo red binding ability, haemolytic activity and the public health significance of isolates especially their role in human diseases were discussed. Twenty different biotypes of E.coli were obtained on the basis of fermentation reactions of six different sugars viz., salicin, starch, sucrose, raffinose, dulcitol and rhamnose. Similarly on the basis of serotyping 80 typable isolates were distributed into 35 different serogroups (08, 01, 0101, 02, 09, 051, 05, 013, 032, 036, 039, 088, 015, 060, 078, 0153, 06, Oil, 023, 025, 027, 029, 050, 058, 061, 068, 076, 081, 086, 0109, 0123, 0133, 0158, 0168 and 0172 in descending order of frequency) along with 10 rough and 10 untypable strains. Majority of the isolates were found sensitive to chloramphenicol (94.00 %) followed by ciprofloxacin (93.00 %), enrofloxacin (88.00 %), gentamicin (84.00 %), co-trimoxazole (65.00 %), furazolidone (40.00 %), ampicillin (12.00 %) and tetracycline (5.00 %) in decreasing order of sensitivity. A total of 63 E.coli isolates were found positive for Congo red binding ability whereas 48.00 per cent isolates revealed presence of haemolytic activity. Among all five shops, highest prevalence oi E.coli was found in shop No.2 (89.99%) and minimum in shop No.4 (50.00 %) and rest three shops ranged in between these two limits.
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VETERINARY PUBLIC HEALTH, A STUDY