Genetic analysis and molecular mapping of components of resistance to shoot fly in maize
| dc.contributor.advisor | Vikal, Yogesh | |
| dc.contributor.author | Arshpreet Kaur | |
| dc.date.accessioned | 2017-02-15T10:39:08Z | |
| dc.date.available | 2017-02-15T10:39:08Z | |
| dc.date.issued | 2016 | |
| dc.description.abstract | Shoot fly (Atherigona naqvii) is a major insect of maize causing yield losses up to 45 per cent. The insect resistance in maize is complex and quantitative in nature. Therefore, the present study was undertaken using 107 F2:3 families from the cross between CM143 as the resistant (female) parent and CM144 as the susceptible (male) parent and data was recorded at different time intervals of inoculation. The disease reaction data showed normal distribution clearly indicating that the inheritance of shoot fly resistance in CM143 is under the control of quantitative loci. F2:3 families alongwith parents were evaluated for various component traits (like deadheart, oviposition, leaf injury, leaf dimensions, seedling vigor, leaf glossiness etc.) that could be involved in shoot fly resistance. Parental polymorphism survey was done with 643 SSR markers and 195 were found to be polymorphic. A linkage map of 1181.11 cM length was constructed with 105 polymorphic SSR markers on 107 F2 individuals. Both genotypic data and mean values of phenotypic data of each component trait was analysed using QTL cartographer and QTLs on chromosome 1, 2, 4 and 9 were detected for various component traits explaining phenotypic variance from 2.15 to 19.43 per cent with LOD score ranging from 2.6 to 5.83. The QTL detected on chromosome 9 for oviposition and deadheart in the present study is syntenic to regions of chromosome 10 of sorghum which was also accounted for deadheart and oviposition. Fine mapping of identified QTL regions may provide useful information of genes underlying quantitative shoot fly resistance that can be further used in for genetic and breeding studies. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810001062 | |
| dc.keywords | Atherigona naqvii, maize, QTLs, shoot fly | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 70 | en_US |
| dc.publisher | Punjab Agricultural University, Ludhiana | en_US |
| dc.research.problem | Genetic analysis and molecular mapping of components of resistance to shoot fly in maize | en_US |
| dc.sub | Agricultural Biotechnology | en_US |
| dc.subject | sowing, intercropping, wheats, tillage equipment, seed drilling, crops, mustard, fertilizers, land resources, chickpeas | en_US |
| dc.theme | shoot fly in maize | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Genetic analysis and molecular mapping of components of resistance to shoot fly in maize | en_US |
| dc.type | Thesis | en_US |