CLINICO-BIOCHEMICAL AND MOLECULAR INVESTIGATIONS OF HAEMOLYTIC ANAEMIA IN DOGS

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dc.contributor.advisorUsha Narayana Pillai
dc.contributor.authorARUN GEORGE
dc.date.accessioned2023-02-16T05:48:23Z
dc.date.available2023-02-16T05:48:23Z
dc.date.issued2022-01-01
dc.descriptionSubmitted in partial fulfillment of the requirement for the degree of Doctor of Philosophy in Clinical Veterinary Medicineen_US
dc.description.abstractHaemolytic anaemia is a cause of concern in all breeds of dogs. Dogs brought to the University Veterinary Hospitals at Mannuthy and Kokkala in Thrissur district were screened for haemolytic anaemia. One hundred and one dogs with haemolytic anaemia were subjected to detailed clinical, biochemical, and molecular investigation. The primary reasons for haemolysis were blood parasites, oxidative injury, and micro-angiopathic haemolytic anaemia and these causative factors occurred as single-etiology or multi-etiology cases. Among the blood parasites, Babesia gibsoni was the major reason for haemolysis followed by Babesia canis vogeli and Ehrlichia canis. Trypanosoma evansi and Anaplasma platys appeared as co-infection in a few cases. Other minor reasons for haemolysis were immune-mediated haemolytic anaemia, stomatocytosis, and an unidentified bacterial organism. Thrombocytopenia was a consistent finding in all haemolytic cases. Eighty-one per cent of dogs with haemolytic anaemia were non-regenerative. Serum phosphorus, total cholesterol and LDL-cholesterol were not significantly different from the healthy controls at p<.05 and did not appear to influence erythrocyte membrane stability as determined by erythrocyte osmotic fragility test and erythrocyte sodium dodecyl stability test. The values of erythrocyte malondialdehyde, reduced glutathione and glutathione peroxidase were not significantly (p<.05) different from the healthy controls. The major erythrocyte morphological abnormalities identified were anisocytosis, microcytosis, hypochromasia, schistocytes, and blister cells. Genotyping of the pre-documented mutations in erythrocyte pyruvate kinase gene, phosphofructokinase gene, α spectrin and β-spectrin genes did not reveal any mutation. To conclude, the major reasons for haemolytic anaemia in dogs were blood parasites, oxidative injury, and microangiopathic haemolytic anaemia, whereas the minor reasons were IMHA, stomatocytosis and bacterial infection. Anti-rabies and multi-component vaccinations, deworming, serum phosphorus, total cholesterol and serum LDL cholesterol did not increase fragility of erythrocytes. The pre-documented gene mutations in erythrocyte pyruvate kinase enzyme, erythrocyte phosphofructokinase enzyme, α-spectrin and β-spectrin proteins were not detected in this study.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810193877
dc.keywordsCLINICO-BIOCHEMICAL AND MOLECULAR INVESTIGATIONS OF HAEMOLYTIC ANAEMIA IN DOGS, INTRACORPUSCULAR FACTORS, Erythrocyte enzymopathies, Pyruvate kinase deficiency, Phosphofructokinase deficiency, Membrane cytoskeletal defects, Stomatocytosis, Elliptocytosis, Spherocytosis, Membrane transport defects, EXTRACORPUSCULAR FACTORS, Oxidative injury, Immune mediated haemolytic anaemia, Heinz body haemolytic anaemia, Infectious agents, Factors affecting erythrocyte membrane stability, Canine Coomb’s test, Extraction of genomic DND from whole blood, Genotyping of pyruvate kinase L/R (PKLR) gene variatns, Genotyping of phosphofructokinase-M (PFKM) gene variant, Genotyping of erythrocyte cytoskeletal gene variants, . Genotyping of alpha-spectrin (SPTA) and beta- spectrin (SPTB) gene variantsen_US
dc.language.isoEnglishen_US
dc.pages221p.en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES THRISSUR, KERALAen_US
dc.research.problem1. Screening of dogs for haemolytic anaemia 2. Investigation on etiology of haemolytic anaemia in dogs 3. Assessment of risk factors for haemolytic anaemia in dogs 4. Assessment of response to treatmenten_US
dc.subClinical Veterinary Medicine, Ethics and Jurisprudenceen_US
dc.themeCLINICO-BIOCHEMICAL AND MOLECULAR INVESTIGATIONS OF HAEMOLYTIC ANAEMIA IN DOGSen_US
dc.these.typePh.Den_US
dc.titleCLINICO-BIOCHEMICAL AND MOLECULAR INVESTIGATIONS OF HAEMOLYTIC ANAEMIA IN DOGSen_US
dc.typeThesisen_US
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