Phytochemical investigation of citrus sinensis var. jaffa and its antifungal activity against fusarium oxysporum
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Date
2007
Authors
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Publisher
CCSHAU
Abstract
Citrus sinensis (L) Osbeck (Syn C. aurantium L. var. sinensis) belongs
to family Rutaceae. It is widely cultivated all over the world. It is known for
its antimicrobial, Insecticidal and medicinal properties. Fruits of C. sinensis
var. Jaffa were collected from Department of Horticulture, CCS HAU, Hisar.
Rinds were separated from fruits and extracted with hot methanol. Column
chromatography of the extractives over silica gel afforded two compounds.
One of these was β-sitosteryl- β-D-glucoside and other was 5-hydroxy-3', 4’-
dimethoxy flavanone 7-O-rhamnosyl (14) glucoside. Both these compounds
are reported 1st time from this plant.
Column chromatography of ethyl acetate extract of juice afforded only
one compound i.e. 5-hydroxy -3', 4'-dimethoxy flavanone 7-O-rhamnosyl (1
4) glucoside. The characterization of isolated compounds was carried out on
the basis of UV-visible, IR, 1H NMR, Mass spectral data and other properties
of the compounds.
The methanolic extract of flavido obtained from C. sinensis var. Jaffa
was used for antifungal activity at 500, 1000, 1500 and 2000 μg/ml
concentration against Fusarium oxysporum and percentage inhibition was
recorded. It was noted that the growth of Fusarium oxysporum was inhibited
to the maximum extent at concentration 2000 μg/ml with 53% inhibition.
Tannins, total sugars, titrable acidity, TSS, moisture content and
ascorbic acid contents (Biochemical parameters) were estimated in the juice of
C. sinensis var.Jaffa. The juice was found to contain total sugars (10.45 g/100
ml), tannins (78.00 mg/100ml), ascorbic acid (47.90 mg/100ml), TSS
(11.55%), moisture content (81.44%) and titrable acidity (1.14 g/100ml).
Description
Keywords
Aromatic compounds, Fruits, Organic acid salts, Alcohols, Acidity, Extraction, Byproducts, Planting, Solutes, Sugar