Selection of parents for yield improvement in Linseed (Linum usitatissimum L.) under irrigated condition
Loading...
Files
Date
2017-08
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Department of Plant Breeding and Genetics, BAU, Sabour
Abstract
Linseed (Linum usitatissimum L.) commonly known as flax having 2n = 30 chromosomes is a self pollinated crop that belongs to the family Linaceae. It is cultivated mainly for its seed oil and its high quality stem fibre. Linseed oil is the richest source of omega-3 fatty acids which has been functionally associated with numerous positive health claims in human and animal. Inspite of all these nutritional value, Linseed is always neglected due to its poor yield and non-availability of improved cultivars which is one of the limiting factors of its popularity. Therefore , a study was undertaken for evaluation of the 31 linseed genotypes collected from AICRP, linseed, Sabour and PC Unit, linseed Kanpur, including two checks, T-397 and Shekhar. The genotypes were grown in randomized complete block design with three replications during Rabi 2015-16 at Bihar Agricultural college farm, BAU, Sabour, Bhagalpur to select parents for yield improvement in linseed. The analysis of variances for design of experiment revealed highly significant differences among the genotypes for all the 20 characters studied. The characters namely, number of primary branches per plant, number of capsules per plant, bud fly infestation, 1000-seed weight, biological yield per plant, seed yield per plant, harvest index, stearic acid and linoleic acid exhibited high GCV, PCV and high heritability coupled with high genetic advance. Correlation studies showed a significant and positive correlation between seed yield per plant and number of capsules per plant, number of seeds per capsule, 1000-seed weight, seed length and biological yield per plant while bud fly infestation was significant and negatively correlated with seed yield per plant. A non significant correlation between oil and fatty acids were found in biochemical study. While, a significant and positive correlation between palmitic acid and stearic acid and negative correlation between stearic and linoleic acid and between oleic acid and linolenic acid were found in present study. Path analysis indicated that bud fly infestation exhibited high and negative direct effect on seed yield per plant. While, number of seeds per capsule and 1000-seed weight showed moderate direct effects on seed yield per plant. Indirect effects of number seeds per capsule, seed length, 1000-seed weight and biological yield per plant on seed yield per plant via bud fly infestation were recorded moderate to high. On the other hand, all the fatty acid namely, stearic acid, oleic acid, linoleic acid and linolenic acid showed high and negative direct effect on oil content except palmitic acid which showed positive and low direct effect. The indirect effect of individual fatty acid via other fatty acids on oil content was recorded moderate to very high. On the basis of D² analysis, 31 genotypes were grouped into 6 divergent clusters. Cluster I was the biggest with 12 genotypes followed by cluster II and cluster V. Inter cluster D2 value was the highest between cluster II and VI followed by V and VI. Molecular diversity analysis was also done with 20 SSR primers. Out of 20 primers, 15 primers were found polymorphic, 3 monomorphic and other two gave non scorable band. The PIC value of SSR primers ranged from 0.1-0.447 with an average of 0.245. However, the similarity coefficient ranged from 0.5 to 0.9. Among the 31 genotypes, the lowest similarity coefficient was observed between GS-202 and Neelum (0.50) followed by the pair EC-1424 and H-40 (0.51) indicating their distant relationship and the highest between Meera and EC-537911 (0.90) indicating presence of a common ancestry. Based on the D2 values, clusters mean and means of the genotypes, nineteen genotypes were selected and their possible cross combinations namely, EC-1424 with BRLS -101, BRLS-102, BRLS-103 for oil & oil quality and EC-1424 with Parvati, EC537911A and Neelum for seed yield and its attributing characters were identified to obtain better segregants in linseed breeding programme.
Description
Keywords
null