EXPERIMENTAL OESOPHAGEAL ANASTOMOSIS BY EVERSION TECHNIQUE IN BUFFALO CALVES (BUBALUS BUBALIS) A HISTOMORPHOLOGICAL AND HISTOCHEMICAL STUDY
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Date
1980
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AAU, Anand
Abstract
Experimentally end-to-end cervical oesophageal anastomosis was performed by everting suture pattern in 12 healthy buffalo oalves, under local ioifiltration anesthesia. A 3 cm of the oesophagus was resected and anastomosed with horizontal mattress sutures using 4/0 chromic cat-gut,. Clinical, radiographic, histomorphological and histoehemieal evaluation of the healing process was done in six animals (group-I) on l4th post-operative day and in the remaining six animals (group-II) on 21st post-operative day. The recovery in all the animals was uneventful. Postnecroptic radiographic study revealed narrowing of the lumen on the 14th day which returned to that of near normal by 21st day, but showing a slight constriction at the site of anastomosis. On 14th post-operative day routine Hematoxylin and Eosin Staining of the oesophageal tissue revealed completion of epithelization but without papillation. However, the gap of the anastomosed tissue was filled by granulation tissue. The cat-gut was still unabsorbed. Verhoeff's staining indicated less of collagen fibres than normal and very few elastic fibres. A similar increase in alkaline phosphatase activity could be demonstrated through modified Gomori's staining. Further, the submucous layer was moderately positive for PAS reaction. By 21st day the papillation of the mucous layer was almost re-established, fhe anastomosed site was bridged by mature fibrous tissue. The cat-gut was seemed to be absorbed. There was evidence of maturity of the collagen fibres. However, the elastic fibre had reappeared and could be compared to that of normal. The alkaline phosphatase activity had returned to the of normal. Similarly, PAS reaction also had almost returned to normal.
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VETERINARY SURGERY, A STUDY