Effect of entomopathogenic fungi on sucking pests and leaf feeders of vegetables under in vitro conditions
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Date
2002
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Department of Agricultural Entomology, College of Agriculture, Vellayani
Abstract
Investigation on "Effect of entomopathogenic fungi on sucking
pests and leaf feeders of vegetables under in vitro conditions" was
carried out II1 the Insect Pathology Laboratory, Department of
Agricultural Entomology. College of Agriculture, Vellayani.
Among the seven entomopathogenic fungi tested, Be auver ia
r a l it ura and
Peri call ia ricini, an aphid isolate of Fusarium pallidoroseum infected
aphids Aphis craccivora and Myzus pers icae while Rhizop us oryzae
caused mortality of Dysdercus cingula/us.
The LCso values for B. bassiana were calculated as S.75 x 108.
6.99 X J08 and 2.72 x 105 spores m]"1 against S. derogat a. S. l it ura and
P. rici ni respectively. F. pallidoroseum caused SO per cent mortality of
A. cracci vora and M. persicae at 3.86 x 106 and 0.72 x 106 spores 111]"1
respectively. R. oryz ae caused 50 per cent mortality of D. cingula/us at
4.15 x 106 spores mrl.
Evaluation of different naturally available substrates for mass
multiplication of B. bassiana. F. p all i dor o se um and R. oryz ae revealed
that both rice bran and gingelly oil cake were suitable for the mass
multiplication of these fungi.'Among the liquid substrates. coconut water
produced maximum mycelial growth of all the fungi.
At room temperature. B. bassiana retained the vi.ulerice up to five
months after inoculation in rice bran and gingelly oil cake. hut the
vi rulence of the fungus was drastically reduced after two months or
storage. F. p al lidorosetnn retained the virulence upto six months in rice
bran and gingelly oil cake but R. oryzae lost the virulence after two
months of storage. Coconut water and starch solution could not retain the
\ i a h i lit y all d vir u I e n c e 0 f the fu n g i a ft e r t w 0 III 0 nth s 0 f s tor age.
l-, p allidoroseum and R. OITZ(/(! with different pesticides revealed that
two per cent neem oil emulsion could be incorporated with B. bassiana
and R. oryzae without any inhibitory effect while sporultio n of
F. pallidoroseum was inhibited by nee m oil. Mancozeb, dimethoate
malathion, quinalphos and chlorpyriphos greatly inhibited the growth
and sporulation of the three fungi tested.
Description
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Citation
171953