ASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN THE QUANTITATIVE TRAIT LOCI WITH RESISTANCE OR SUSCEPTIBILITY TO TICK INFESTATION IN CATTLE

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dc.contributor.advisorDinesh, C.N.
dc.contributor.authorBHAGYASHREE MUKUND, KAMBLE
dc.date.accessioned2021-01-13T10:13:06Z
dc.date.available2021-01-13T10:13:06Z
dc.date.issued2019-10-05
dc.description.abstractThe present study was aimed to identify the polymorphisms in five QTLs that were reported to have an association with resistance/susceptibility to tick infestation in cattle and to find out the association of SNPs with tick infestation in cattle. It was also intended to study the effect of season on the association between the SNPs and tick infestation in cattle. The population under study comprised 45 Vechur and 74 crossbred cattle from the farms of Kerala Veterinary and Animal Sciences University. The QTLs and SNPs were selected from the QTL database, Animal QTLdb. The SNPs and the QTLs (in parenthesis) used in this study were: rs41661020 (#101148), rs109162468 (#101169), rs43708490 (#135798), rs41577070 (#135800) and rs29009970 (#135801). Blood samples were collected from the animals and the genomic DNA was isolated and used for genotyping and polymorphism study by PCR-RFLP assay and confirmed by sequencing. Genotyping was done by direct counting and the allelic and genotypic frequencies were estimated by POPGENE 1.32. The results revealed a monomorphic pattern for the SNP rs109162468 and polymorphic pattern for the SNPs rs41577070, rs41661020, and SNP rs43708490 in the population under study. The SNP rs29009970 was monomorphic in the crossbred cattle, whereas, dimorphic in Vechur. The Chi-square test revealed that crossbreds and Vechur were in HWE for SNPs rs41577070, rs41661020, and rs43708490, whereas, the SNPs rs29009970 showed a significant departure from the equilibrium. For the association study, tick counts were taken during summer (March to May) and rainy (June to August) seasons from the animals using Wharton and Utech (1970) method. However, no tick infestation could be seen in any animals during the period of study. Hence, all the 119 animals were grouped in the class: no infestation and the analysis to find the association of SNPs with tick infestation and the effect of the season on the SNP-tick load relationship in cattle could not be carried out.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810160053
dc.keywordsCattles, SUSCEPTIBILITY TO TICK INFESTATION, SINGLE NUCLEOTIDE, POLYMORPHISMS, QUANTITATIVE TRAIT LOCIen_US
dc.language.isoEnglishen_US
dc.pages60en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANADen_US
dc.research.problemSINGLE NUCLEOTIDE POLYMORPHISMS IN THE QUANTITATIVE TRAIT LOCI WITH RESISTANCE OR SUSCEPTIBILITY TO TICK INFESTATION IN CATTLEen_US
dc.subAnimal Genetics and Breedingen_US
dc.themeASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN THE QUANTITATIVE TRAIT LOCI WITH RESISTANCE OR SUSCEPTIBILITY TO TICK INFESTATION IN CATTLEen_US
dc.these.typeM.V.Sc.en_US
dc.titleASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN THE QUANTITATIVE TRAIT LOCI WITH RESISTANCE OR SUSCEPTIBILITY TO TICK INFESTATION IN CATTLEen_US
dc.typeThesisen_US
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