Studies on serological response in female calves vaccinated with S-19 Brucella abortus vaccine and molecular diagnosis of brucellosis in cows and buffaloes
| dc.contributor.advisor | Gupta, M. P. | |
| dc.contributor.author | Fareed, Aadil | |
| dc.date.accessioned | 2021-01-04T10:10:10Z | |
| dc.date.available | 2021-01-04T10:10:10Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | Brucellosis is an infectious disease caused by Gram negative facultative intracellular bacterial organisms of the genus Brucella. The total of 479 blood samples were collected to determine the seroprevalence of Brucellosis among 3 different agroclimatic zones of Punjab using Brucella abortus coloured antigen (RBPT). Percent seroprevalence in submontane zone (Zone I), central zone (Zone II) and arid irrigation zone (Zone III) was found to be 17.74, 22.91 and 6.17 respectively The antibodies against Brucella abortus were found to be in 57 out of 264 cattle (21.5%) and 36 out of 215 buffaloes (16.7%). The overall seroprevalence of Brucellosis using RBPT was found to be 19.41%. The incidence of abortion was significantly more (90.32%) in last trimester of pregnancy, while history of repeat breeding was also found to be in 16.39% of Brucella positive animals. 19 female calves vaccinated with Brucella abortus S-19 calfhood vaccine were found to show a serological response of first increase in the antibody titre upto 60th day post-vaccination and then decrease in the titre thereafter using serological tests of STAT and MTPAT. In the second group of 15 adult cows already vaccinated with Brucella abortus S-19 vaccine were found to show negative titre on application of RBPT, STAT and MTPAT after a lapse of 18 months post-vaccination. Out of 40 samples of aborted fetal stomach/abomasal contents and/or vaginal mucus/uterine discharges of recently aborted animals, 11 were found to be positive for Brucellosis on application of molecular diagnostic test of PCR. Out of 20 seropositive heparinised whole blood samples, 4 were found to be positive for Brucellosis on applying PCR. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810159051 | |
| dc.language.iso | English | |
| dc.publisher | GADVASU, Ludhiana | |
| dc.sub | Epidemiology and Preventive Veterinary Medicine | |
| dc.these.type | M.V.Sc. | |
| dc.title | Studies on serological response in female calves vaccinated with S-19 Brucella abortus vaccine and molecular diagnosis of brucellosis in cows and buffaloes | |
| dc.type | Thesis |
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