Molecular profiling of mungbean genotypes vigna radiata (L.) wilczek using RAPD analysis
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Date
2007
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Publisher
CCSHAU
Abstract
A study on 39 genotypes of mungbean representing a cross-section of
variability was undertaken to assess RAPD polymorphism and to estimate the
matrix of Jaccard’s similarity coefficient. A total of 411 amplified products
comprising 382 (92.9%) polymorphic and 29 (7.1%) monomorphics markers
were observed using 30 RAPD primers. The number of bands generated per
primer ranged from 7 to 20 bands with a mean of 13.7 bands per primer. The
primers showed a large variation in the frequency of polymorphic bands (0.026
- 0.974) and in number of banding patterns (3 – 35). Efficiency of primers were
analyzed using Discrimination power (D), Diversity index (DI), Resolving
power (Rp) and Marker index (MI). All efficiency parameters except DI could
identify most efficient (S-1 and S-2) and the least efficient (S-30) primers. Both
discrimination power (D) and resolving power (Rp) parameters appear to be
good measure of the usefulness of primers to identify cultivars. The
experimental results obtained for real efficiency of the primer agreed well with
that of theoretical expectation based on independent hydpothesis of primers.
Genetic similarity coefficient ranged from 40.8 per cent to 90.3 per cent
indicating moderate level of genetic variability among genotypes. Some of the
genotypes such as K 851, Pusa Ratna, MH 98-1, Asha, MH 96-1 Muskan,
Koppergaon, K 851, Pusa Baishakhi, ML 1108, PDM 262, ML 682, Pusa 9072
and ML 818 exhibited higher genetic similarity with rest of the genotypes. ML
131 was the most diverse genotype. Other genotypes namely ML1349, Pant
M-4, Greengram Co-5, SML 32, UP 99-3, ML 1333, UPM-98 also exhibited
higher diversity. The dendrogram constructed from Jaccard’s similarity
coefficient showed three main groups. The group A consists of 26 genotypes
while group B and C consist of three and two genotypes only. The mungbean
genotypes evolved at PAU, Ludhiana (Punjab) and in Uttar Pradesh were
comparatively more diverse among themselves than the group of genotypes
from Hisar (Haryana) and IARI (Delhi). The grouping of some genotypes
corresponded with their pedigree information but failed in other cases. The
same was true with the geographical origin vis-à-vis grouping of genotypes.
Mantel’s test between the cophenetic matrix and the original matrix
(z=r=0.923) revealed a good fit of cluster analysis performed. The pattern of
grouping in cluster analysis was also reflected in Principal Coordinate Analysis
(PCoA) with minor differences. The three coordinate PCoA could account
73.68 per cent of total variability in RAPD markers. The high level of RAPD
polymorphism observed in mungbean may be useful for variety protection and
monitoring the genetic diversity.
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Keywords
Genotypes, Genetics, Rapd, Polymorphism, Dna, Planting, Pcr, Genetic markers, Biological phenomena, Enzymes