IDENTIFICATION AND VALIDATION OF DROUGHT-RESPONSIVE GENES IN THE UPLAND RICE CULTIVAR ‘BANGLAMI

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dc.contributor.advisorModi, M.K.
dc.contributor.authorBorah, Akankshita
dc.date.accessioned2021-01-05T10:33:43Z
dc.date.available2021-01-05T10:33:43Z
dc.date.issued2020-10
dc.description.abstractDrought is one of the most important and highly unpredictable abiotic stresses causing drastic reductions in yield under rainfed rice environments, affecting 20% of the total rice-growing area in Asia (Pandey and Bhandari, 2008). Assam has diverse germplasm of rice which includes Banglami, a traditional drought-tolerant Ahu rice cultivar found locally in the state. Since 2013, crossing has been carried out between this drought-tolerant cultivar and Ranjit, a high yielding but drought-susceptible elite cultivar. Many drought-related QTLs were identified and reported from this cross-population. For the current study, twenty QTLs identified in the F4 population were selected for mapping on the parental genome. The results revealed that 17 of the 20 QTLs were present on the parent genomes where 11 of the 20 QTLs (qEBT3.1, qEBT6.2, qNOT6.1, qNOT2.1, qPL1.1, qPL1.2, qPL9.1, qPL9.2, qPL1.1, qNOG9.1, qNOG12.1) were found common to both the cultivars. From these, two of the QTLs (qGY1.1, qGY7.1) were found to be exclusively contributed by Banglami while four of the QTLs (qRLWC9.1, qRLWC9.2, qPH7.1, qDTF12.2) were found to be contributed by Ranjit to the cross-population. Genes associated with the QTLs were identified from whole-genome sequence data of both the cultivars. In total, 2454 genes were located in the 13 QTLs found in Banglami while 1585 genes were found in Ranjit in the 15 QTLs. The differential expression of the candidate genes in water-stress and irrigated conditions were calculated with the help of available RNA-seq data. Differential gene expression was calculated for each of the candidate genes under drought and control. Fifty DEGs in Banglami and seventy-two DEGs in Ranjit having a Log2 Fold change greater than ± 2.5 up to ± infinity were considered significant. Few of the differentially expressed genes having a significant Log2Fold change as revealed by the sequencing data, were confirmed experimentally under drought situation using qRT-PCR. Stress-responsive candidate genes involved in nitrate uptake, DNA repair, ubiquitination, enzymatic activities, cell cycle activities, etc. which can play a significant role in conferring drought tolerance were identified in the drought-related QTLs. The expression pattern of the candidate genes in qRT-PCR was found similar to transcriptome studies, signifying important role of the candidate genes in drought tolerance mechanism in rice.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810159216
dc.language.isoEnglishen_US
dc.publisherAAU, Jorhaten_US
dc.subAgricultural Biotechnologyen_US
dc.themeIDENTIFICATION AND VALIDATION OF DROUGHT-RESPONSIVE GENES IN THE UPLAND RICE CULTIVAR ‘BANGLAMIen_US
dc.these.typeM.Scen_US
dc.titleIDENTIFICATION AND VALIDATION OF DROUGHT-RESPONSIVE GENES IN THE UPLAND RICE CULTIVAR ‘BANGLAMIen_US
dc.typeThesisen_US
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