EXPERIMENTAL STUDY ON INDUCED TOXICITY OF ARSENIC AND LEAD AND THEIR INTERACTION IN WISTAR RATS

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dc.contributor.advisorJOSHI, B. P.
dc.contributor.authorRAVAL, JEETENDRA KUMAR. K
dc.date.accessioned2018-05-25T05:31:59Z
dc.date.available2018-05-25T05:31:59Z
dc.date.issued2008
dc.description.abstractThe present study has been carried out to study clinical signs, pathomorphological changes, haemato-biochemical alterations, oxidative stress effects as well as genotoxic effect induced by arsenic and lead and their mixture in biological system of rat. Accordingly sixty colony bred albino wistar rats of both sexes, were divided uniformly in to five groups as group I, II, III, IV and V. The group I received only deionised water and served as control while group II,III and IV were orally dosed with sodium arsenite @ 4 mg/kg body weight; lead acetate @ 53 mg/kg body weight;and mixture of sodium arsenite @ 4 mg/kg and lead acetate @ 53 mg/kg respectively for 28 days . The group V served as positive control for genotoxicity study and was given cyclophosphamide( 20 mg/kg body weight) intraperitoneally 24 hour before the terminal sacrifice. The clinical signs like dullness, diarrhoea, decreased appetite, irritability and pale mucous membrane were noticed in all the three experimental groups with varied severity. In addition to this lead treated group showed respiratory distress while mixture treated group revealed distended abdomen. Sodium arsenite , lead acetate and mixture of both when orally dosed to rats, showed decrease in body weight and feed consumption as compared to control with maximum decrease in group IV. The water intake remained unaffected in all the treatment groups. There was significant reduction in TEC and Hb in all the treatment groups suggestive of anemia and when arsenic and lead given in combination resulted in macrocytic anemia. Mixture of arsenic and lead as well as lead as single metal alone has resulted in leucopenia due to lymphopenia and relative neutrophilia. Increase in AKP, GGT, creatinine and decrease in TP and albumin revealed hepatic and renal damage and were marked more with mixture of both the metals as compared to single metal alone. There was significant increase in LPO and decrease in SOD in all the three treatment groups. Difference in values of SOD and LPO as compared to control was highest in group IV followed by group III and II indicating oxidative stress more pronounced with mixture of both metals. Pathomorphological lesions induced by arsenic and lead as well as their mixture in organs like liver, kidney, lung, intestine and testis were of the nature of vascular, degenerative and inflammatory changes with varied severity and extent of distribution. The overall lesions gave impression that both the metals arsenic and lead were hepatotoxic as well as nephrotoxic in nature. The severity of lesions in different organs was more with mixture of both the metals. All the three treatment groups showed increase in micronuclei and chromosomal aberrations as compared to control. Arsenic and lead when administered alone were equally genotoxic to that of mixture of both metals at the given dose level while their genotoxic potential was less than the known positive compound cyclophosphamide.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810047504
dc.keywordsEXPERIMENTAL STUDY ON INDUCED TOXICITY, ARSENIC AND LEAD, THEIR INTERACTION IN WISTAR RATSen_US
dc.language.isoenen_US
dc.publisherAAU, Ananden_US
dc.research.problemEXPERIMENTAL STUDY ON INDUCED TOXICITY OF ARSENIC AND LEAD AND THEIR INTERACTION IN WISTAR RATSen_US
dc.subVeterinary Pathologyen_US
dc.subjectVETERINARY PATHOLOGYen_US
dc.subjectAn Experimental Studyen_US
dc.themeEXPERIMENTAL STUDY ON INDUCED TOXICITY OF ARSENIC AND LEAD AND THEIR INTERACTION IN WISTAR RATSen_US
dc.these.typeM.V.Sc.en_US
dc.titleEXPERIMENTAL STUDY ON INDUCED TOXICITY OF ARSENIC AND LEAD AND THEIR INTERACTION IN WISTAR RATSen_US
dc.typeThesisen_US
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