“ISOLATION OF PPR VIRUS, DETECTION BY ELISA AND DIFFERENT GENE BASED RT-PCR”
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Date
2008
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Publisher
Anand Agricultural University
Abstract
Peste des petits ruminants (PPR) is a severe viral disease of goats and
sheep with high morbidity and sometimes high mortality characterized by
fever, erosive stomatitis, conjunctivitis, gastroenteritis and pneumonia. PPR is
caused by Peste des petits ruminants virus (PPRV), a Paramyxovirus of the
Morbillivirus genus. The disease causes severe economic losses to small
ruminant husbandry and is presently considered as one of the major threats to
about 200 million small ruminant population of the country. The present study
was aimed to detect PPRV in clinical samples using s-ELISA and to derive
estimates of overall, locationwise and specieswise incidence of PPRV. The
study also involved the isolation of PPR virus from clinical samples and
assessment of F, N and H gene targets for detection of PPRV by RT-PCR from
Description
Keywords
Veterinary Microbiology, Analysis