COMPARATIVE EVALUATION OF DIFFERENT ESTRUS INDUCTION AND SYNCHRONIZATION PROTOCOLS IN ANOESTRUS COWS AND BUFFALOES
Loading...
Date
2015
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
AAU, Anand
Abstract
This investigation was aimed to evaluate the clinical response and monitor the peripheral plasma progesterone, biochemical and macro-minerals profiles in postpartum anoestrus crossbred cows and buffaloes treated with different standard hormonal protocols (TriU-B/PRID, Ovsynch and Heatsynch), keeping untreated anoestrus control and normal cyclic control groups. The study was carried out in the villages of Anand and Mahisagar districts of Gujarat as well as Livestock Research Station of NAU, Navsari. The work was carried out on 92 true anoestrus cows and buffaloes (46 each) with more than 90 days postpartum period, and 20 normal cyclic control cows and buffaloes (10 each) that exhibited spontaneous estrus within 90 days postpartum. The animals selected were followed for a period of 3 months post-treatment/post-breeding. All infertile animals identified were dewormed using Inj. Ivermectin @ 100 mg s/c and were also treated once initially with i/m injection of inorganic phosphorus (Inj. Alphos- 40 @ 10 ml, Pfizer Animal Health) and multivitamins AD3E (Inj. Vetacept @ 10 ml. Concept Pharma) and were supplied with multi-minerals bolus (Minotas, Intas Pharma) @ 1 bolus daily for 7 days. The effect of three hormonal protocols was evaluated by comparing the estrus induction response, estrus induction interval and induced/first estrus (with fixed time AI) as well as overall of three cycles' conception rates, and monitoring plasma progesterone (by RIA), total' cholesterol, total protein, calcium and inorganic phosphorus (by autoanalyzer) profiles at different time intervals (day 0, 7, 9/10-AI) of treatment and day 21 post-FTAI in anoestrus buffaloes, and on day of estrus/AI and day 21 post-AI in anoestrus control and normal cyclic control groups. Twelve true anoestrus crossbred cows and buffaloes each (Gr.-I) were treated with TriU-B (0.96 g progesterone in elastic rubber moulded over a cross "S" shaped nylon spine, Virbac) intravaginally for 7 days with a regular 1/m dose of 500 μg PGF2α (Repregma 2 ml, Vet Mankind) o n the sixth day, and estradiol benzoate 0.75 mg (Sigma) on 7th day while removing the insert and FTAI was done twice on day 8 and 9. Similarly, 12 true anoestrus cows and buffaloes each in Gr.-II (Ovsynch protocol) were administered with i/m Inj. of Buserelin acetate 10 μg (GnRH analogue, Inj. Ovulanta @ 2.5 ml. Vet Mankind) on day 0, Inj. PGF2α 500 μg (Inj. Repregma @ 2 ml) on day 7, and second injection of Buserelin acetate 10 μg (Ovulanta @ 2.5 ml) on day 9, with FTAI on day 9 and 10, and in Gr.-III (Heatsynch protocol) 12 anoestrus cow and buffaloes each were administered with i/m injection of Buserelin acetate 10 µg (Inj. Ovulanta @ 2.5 ml) followed by injection PGF2α 500 μg (Repregma @ 2 ml) and estradiol benzoate 1 mg (Sigma) on days 0, 7 and 8, respectively, with FTAIs on day 10 M/E using good quality frozen-thawed semen.
Description
Keywords
ANIMAL REPRODUCTION, GYNAECOLOGY AND OBSTETRICS, EVALUATION