MOLECULAR GENETIC STUDY OF LEPTIN GENE IN GIR CATTLE AND MEHSANA BUFFALO
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Date
2008
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Anand Agricultural University, Anand
Abstract
Leptin is a 16-kDa protein synthesized by adipose tissue and is involved in
regulation of feed intake, energy balance, fertility, and immune functions. Leptin binds to
a receptor mainly localized on neuropeptide Y-neurons. The bovine leptin gene is
organized into three exons separated by two introns, of which only 2 exons (exon-2 and
3) are translated into proteins. Variation in the exonic region of a gene may lead to
changes in amino acids which alter the expressed protein. Therefore, the present study
was undertaken to detect polymorphism at respective restriction sites in Leptin gene
exon-3 by PCR-RFLP, SSCP, and sequencing in Gir cattle and Mehsana buffalo breeds