Management of bitter gourd mosaic by enhancing host resistance
| dc.contributor.advisor | Vimi, Louis | |
| dc.contributor.author | Ashwini, K N | |
| dc.contributor.author | KAU | |
| dc.date.accessioned | 2022-10-17T09:21:27Z | |
| dc.date.available | 2022-10-17T09:21:27Z | |
| dc.date.issued | 2015 | |
| dc.description | PG | en_US |
| dc.description.abstract | Bitter gourd (Momordica charantia L.) is one of the important vegetable crops that occupy a pivotal position among fruit vegetables, particularly in south India. The fruits of this crop which have high commercial value and are being used for culinary preparations and various medicinal preparations. In spite of the economic importance of this vegetable, the research work carried out on protection of crop from viral disease is quite scanty. In many case, cent per cent mosaic incidence was recorded in the crop resulting in substantial economic loss. So the present study was focused on screening of bitter gourd accessions and management of bitter gourd mosaic by enhancing host resistance using defense inducers. The three different viruses causing mosaic in bitter gourd are cucumber mosaic virus (CMV), potyvirus and bitter gourd distortion mosaic virus (BDMV). As these viruses causes mixed infection in field, the separation of individual viruses was carried out using systemic indicator host plants. For separation of CMV and potyvirus, systemic indicator host plants used were cosmos and papaya respectively. BDMV was separated by white fly transmission. The pure cultures of viruses were maintained on the susceptible bitter gourd variety Preethi. The symptoms developed by different viruses were recorded under natural and artificial conditions were recorded CMV produced mosaic specks, yellow-green mosaic patches, leathery leaves and downward rolling of leaf margin. Symptoms of potyvirus infection were vein clearing, puckering, malformed leaf with reduced leaf size and rugosity. BDMV infection produced mosaic, puckering, leaf distortion, hairy growth on leaves and vines with reduction in leaf size and internodal length. For the screening of bitter gourd accessions against CMV and potyvirus, potassium phosphate buffer pH 7.0 was found to be the most suitable buffer. Among 22 accessions screened, three accessions viz., TCR 285, TCR 39 and TCR 53 were highly resistant to CMV; one accession Biliagala was highly resistant to potyvirus and 11 accessions viz.,TCR 285, TCR 39, TCR 493 ,TCR 416, TCR 492, TCR 494,TCR 380, TCR 202 and TCR 149, Green long and Biliagala were highly resistant to BDMV. The field experiment was undertaken with the objective of management of bitter gourd mosaic by using defense inducers. The three different defense inducers viz., salicylic acid 25 ppm, barium chloride 0.1% and Pseudomonas fluorescens 2 % were evaluated on the moderately resistant cultivar white long and susceptible variety Preethi. The mosaic symptom was recorded after 51 days of sowing in salicylic acid treated plants and after 40 days of sowing in control. A time gap of 5-10 days after spray of defense inducer was required for development of resistance in plants. The lowest disease severity was observed in cultivar White long treated with salicylic acid. The highest yield was recorded in Preethi treated with Pseudomonas fluorescens. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810189041 | |
| dc.keywords | Bitter gourd mosaic, CMV infection in bitter gourd ,Potyvirus infection in bitter gourd, BDMV infection in bitter gourd ,Separation of virus cultures, Maintenance of virus culture, Bitter gourd | en_US |
| dc.language.iso | English | en_US |
| dc.publisher | Department of Plant Pathology, College of Horticulture, Vellanikkara | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.theme | host resistance | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Management of bitter gourd mosaic by enhancing host resistance | en_US |
| dc.type | Thesis | en_US |