FREEZABILITY OF MALABARI BUCK SEMEN SUPPLEMENTED WITH CURCUMIN AND ASCORBIC ACID
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Date
2017
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD
Abstract
The aim of this study was to determine the cryopreservation induced oxidative
damages and the effects of curcumin and ascorbic acid supplementation on post thaw
quality of Malabari buck semen. Sixty ejaculates were collected from five adult Malabari
buck were used for the study. After removal of seminal plasma, the sperm pellets in Tris
buffer were pooled and then divided into five equal parts. The semen sample belonging to
C-1 and C-2 were extended with Tris based extender containing 0.5 mM and 2.5 mM
curcumin, respectively. Semen samples of A-1 and A-2 were extended with Tris based
extender containing 2.5 mg/ml and 8.5 mg/ml ascorbic acid, respectively and the final
group without antioxidants as control. The extended semen was packed in French mini
straws and manual freezing was carried out after an equilibration period of two hours at
5°C.
The pre freeze motility and viability was found to significantly (p<0.01) higher in
groups with 0.5 mM curcumin and 2.5 mg/ml ascorbic acid than that of control. There was
no significant difference (p>0.01) in sperm morphological abnormalities, malondialdehyde
concentration and DNA integrity between the groups. A significantly (p<0.01) higher
percentage of acrosome integrity was observed with 2.5 mg/ml ascorbic acid
supplementation. The functional membrane integrity was significantly higher in groups
with 2.5 mM curcumin and 2.5 mg/ml ascorbic acid.
The supplementation of 0.5 mM curcumin and 2.5 mg/ml ascorbic acid resulted in
significantly (p<0.01) higher post thaw motility and viability. The post thaw sperm
abnormality per cent with supplementation of 8.5mg/ml ascorbic acid was significantly
(p<0.01) higher than that of other treatment groups and control. The groups containing 2.5
mM curcumin and 2.5 mg/ml ascorbic acid showed significantly (p<0.01) higher
functional membrane integrity. Acrosome integrity was found to be significantly (p<0.01)
higher with 2.5 mg/ml ascorbic acid supplementation whereas 2.5 mM curcumin
supplementation significantly reduced the malondialdehyde production than the other
groups. There was no significant difference (p>0.01) in the DNA integrity with
supplementation of antioxidants than that of control.
Thus supplementation of 2.5 mg/ml ascorbic acid resulted in better post thaw
sperm quality. The two curcumin supplemented groups showed different effect on the
sperm parameters under study. Further in vivo fertility trial are required before
recommending 2.5 mg/ml ascorbic acid for field level artificial insemination.
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