FOLLICULAR DYNAMICS AND EXPRESSION PROFILE OF B-CELL LYMPHOMA-2 GENE IN MALABARI DOELINGS SUPPLEMENTED WITH MORINGA OLEIFERA LEAVES
| dc.contributor.advisor | Dr. Shibu Simon | |
| dc.contributor.author | AMRITHA ARAVIND | |
| dc.date.accessioned | 2024-05-14T11:36:56Z | |
| dc.date.available | 2024-05-14T11:36:56Z | |
| dc.date.issued | 2022-06-22 | |
| dc.description.abstract | The study was conducted to assess the effect of of M. oleifera leaves on reproductive performance in Malabari doelings. Apparently healthy Malabari kids (n= 32) of age three months were selected and randomly allocated to two different treatment groups. Control group (T1, n= 16) was fed with complete feed as pellets and experimental group (T2, n=16) was given complete feed in which 50 per cent of concentrate was replaced with M. oleifera leaves on dry matter (DM) basis. The feeding trials were continued till two oestrous cycles were completed. The mean initial (T1-8.00 ± 0.50 kg; T2- 7.89 ± 0.55 kg) and final body weights (T1-18.73 ± 0.89 kg; T2- 19.95 ± 0.89 kg) did not differ significantly whilst showed a linear increase in both the groups. Daily DMI and haematological parameters (Hb, Haematocrit, TEC, TLC) did not vary significantly between groups. In T2, the mean total protein was significantly higher (p < 0.05) while mean ALT remained significantly lower (p < 0.05). Post weaning mean progesterone profile remained less than 1ng/mL until fifth month of age but showed significant increase during sixth month (T1- 0.24 ng/mL, T2- 0.44 ng/mL; p < 0.05) and elevated more than 1ng/mL during seventh month in T2 (T1- 0.72 ± 0.22 ng/mL, T2- 1.14 ± 0.29 ng/mL). First behavioural oestrus was observed during sixth month age (T1-12.50 %; T2- 31.25 %) without any significant variation between groups. On ultrasound examination of follicular dynamics in two consecutive oestrous cycles, significantly higher number of small, medium and large follicles could be observed in T2 (p < 0.01). Typical wave patterns of one to five wave cycles could be noticed in Malabari doelings in peripubertal period regardless the experimental groups and short cycles were characteristic of first oestrous cycle in both the groups. The first interwave interval of five wave cycle and ovulatory wave of four wave cycle was longer in T2. The two groups did not differ significantly with regard to diameter of largest follicles in each wave, day of appearance of largest follicle, day of ovulation, number and size of ovulatory follicles. The size of corpus luteum, blood flow assessed by pixel count and mean progesterone values were higher during luteogenesis of first oestrous cycle in T2 (p < 0.05). Significantly higher behavioural and physiological score and oestrus intensity could be observed in T2 (p < 0.01). Length of oestrous cycle and duration of oestrus did not vary significantly between the groups. Following follicular aspiration (n=6), significantly higher (p < 0.05) culture grade (A and B) oocytes could be observed in T2 (85.71%) than T1 (43.75%). BCB staining revealed, significantly higher (p < 0.05) number of oocytes with developmental competence in T2 (3.83 ± 0.48) than T1 (2.17 ± 0.48). Follicular health assessed by relative expression of antiapoptotic gene BCL-2 showed no significant variation between the groups. Thus, the present study authorizes the effective and safe means of replacement of 50 per cent concentrate ration of goats with M. oleifera leaves without affecting the growth, dry matter intake and haematobiochemical profiles in Malabari doelings and it improved the reproductive performance by advancing the puberty, folliculogenesis, luteogenesis and enhanced developmental competence and quality of oocytes during peripubertal period. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810208949 | |
| dc.keywords | FOLLICULAR DYNAMICS AND EXPRESSION PROFILE OF B-CELL LYMPHOMA-2 GENE IN MALABARI DOELINGS SUPPLEMENTED WITH MORINGA OLEIFERA LEAVES | |
| dc.keywords | FOLLICULAR DYNAMICS | |
| dc.keywords | B-CELL LYMPHOMA-2 GENE | |
| dc.keywords | MORINGA OLEIFERA LEAVES | |
| dc.language.iso | English | |
| dc.pages | 214 | |
| dc.publisher | COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, KERALA VETERINRY AND ANIMAL SCIENCES UNIVERSITY | |
| dc.research.problem | Follicular activity and oocyte quality , Expression profile of B-cell lymphoma-2 (Bcl-2) gene in granulosa cells of ovarian follicles , Hormonal, haematological and biochemical profiles | |
| dc.sub | Animal Reproduction, Gynaecology and Obstetrics | |
| dc.theme | Follicular dynamics and expression profile of B- cell lymphoma-2 gene in Malabari doelings supplemented with Moringa oleifera leaves | |
| dc.these.type | Ph.D | |
| dc.title | FOLLICULAR DYNAMICS AND EXPRESSION PROFILE OF B-CELL LYMPHOMA-2 GENE IN MALABARI DOELINGS SUPPLEMENTED WITH MORINGA OLEIFERA LEAVES | |
| dc.type | Thesis |