Induction of mutagenesis in Periwinkle (Catharanthus roseus L.) through gamma irradiation
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Date
2022
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Punjab Agricultural University, Ludhiana
Abstract
The present experiment entitled "Induction of mutagenesis in periwinkle (Catharanthus roseus L.) through gamma irradiation" was conducted at Floriculture Research Farm of Punjab Agricultural University, Ludhiana during the year 2020-2021. The seedlings of periwinkle were treated with 0Gy, 25Gy, 50Gy, 75Gy and 100Gy with the objective to analyze the effect of different dose of gamma radiation on plant growth and flowering the different dose of gamma rays in periwinkle. To induce mutation and to analyze the gamma radiation treatment led the significant changes in the agronomical traits such as mortality rate, plant height, plant spread, leaf length, leaf width, leaf area, number of plant per plants, flower color, flower size, leaf color and stem girth etc. The maximum morality was observed at 100Gy i.e 23.52% while it was minimum at 25Gy treatment and in untreated plants. The highest rate of survival was observed in 25Gy treatment (88.24%) and the minimum survival % was recorded at 100Gy treatment (50.99%). The plant height and plant spread decreased significantly with the increase in gamma ray dose and recorded maximum in 25Gy treatment i.e 77.34cm and 90.14cm. Maximum number of primary branches with 25Gy treated plants was increased up to 5.40/plant. The leaf length (6.63cm), leaf width (2.76cm) and leaf area (18.36cm2) was recorded to be maximum under 25Gy treatment. The plants treated with 100Gy treatment took longer time to flowering as compared with lower dose of 25Gy treatment. The flower number decreased significantly with the increase in gamma ray dose and recorded maximum in 25Gy treatment. Slight variation in flower color of plants treated with 75Gy and 100Gy was recorded in three plants and it was Purple (P N 78 B) and Purple (P N 78 C), whereas there is no change in leaf color. The flower size decreased to 4.34cm at higher dose of 75Gy. The optimum dose of gamma rays for induction of mutagenesis for periwinkle is 75Gy and 100Gy.
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Kamarpal Singh (2022). Induction of mutagenesis in Periwinkle (Catharanthus roseus L.) through gamma irradiation (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.