Characterization of psychrotolerant rhizobacteria for Zn biofortification in lentil (Lens culinaris L. Medikus)

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dc.contributor.advisorKhanna, Veena
dc.contributor.authorJaskiran Kaur
dc.date.accessioned2019-02-02T09:03:49Z
dc.date.available2019-02-02T09:03:49Z
dc.date.issued2018
dc.description.abstractSeventy psychrotolerant rhizobacterial isolates were evaluated for their PGP traits at 10°, 20° and 30°C. Highest IAA production was recorded with isolates PRh-9, PRh-14, PRh- 15, PRh-60, PRh-61 and that was confirmed by HPLC (8.98, 4.91, 5.66, 2.73 and 1.62 μg/ml respectively). Isolate PRh-14 recorded maximum Zn-solubilization potential of 354, 304.8 and 284.5 ppm at 10°, 20° and 30°C. It also recorded the maximum P-solubilization efficiency, gibberellins, EPS production and biofilm formation at 10°, 20° and 30°C. Eleven isolates amplified acdS gene which confirmed the presence of ACCD activity. Maximum naringin and quercetin production was shown by isolate PRh-61 and PRh-9, further confirmed by TLC studies. Highest antagonistic activity was recorded by PRh-9 against Rhizoctonia bataticola and Fusarium oxysporum (33.3% and 48.1% respectively) and was also established by SEM analysis. Potent siderophore, salicyclic acid and HCN producers were PRh-9, PRh- 61 and PRh-60. The SEM analysis of root surface revealed that coinoculation with Rhiobium and PRh-14 helped in maintaining the root integrity owing to biofilm formation on the root surface. Under field conditions coinoculation of Rhizobium with PRh-15, PRh-14, PRh-9, PRh-61, PRh-60 and PRh-30 further increased by grain yield by 1.7, 4.4, 3.7, 2.5, 2.09, 1.5% respectively, over Rhizobium alone. A significant increase in the grain and straw zinc content was recorded with the treatment R+PRh-14 (105.9 and 141.0 ppm respectively). The DGGE analysis of soil DNA depicted a composite banding pattern that reflected a high microbial diversity. The promising rhizobacterial isolates were identified as PRh-9 (Pseudomonas flourescens), PRh-14 (Aeromonas hydrophila), PRh-60 (Lysinibacillus fusiformis) and PRh-61 (Pseudomonas korensis) based on 16S rDNA sequencing.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810093381
dc.keywords16S rDNA, HPLC, DGGE, PGP traits, SEM analysis, lentil, Lens culinaris L. Medikusen_US
dc.language.isoenen_US
dc.pages117en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemCharacterization of psychrotolerant rhizobacteria for Zn biofortification in lentil (Lens culinaris L. Medikus)en_US
dc.subMicrobiologyen_US
dc.subjectnullen_US
dc.themeCharacterization of psychrotolerant rhizobacteria for Zn biofortification in lentil (Lens culinaris L. Medikus)en_US
dc.these.typePh.Den_US
dc.titleCharacterization of psychrotolerant rhizobacteria for Zn biofortification in lentil (Lens culinaris L. Medikus)en_US
dc.typeThesisen_US
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