MOLECULAR ANALYSIS OF STAT5A AND PPARGC1A LOCI IN MEHSANA BUFFALOES (Bubalus bubalis
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Date
2008
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Anand Agricultural University
Abstract
India has emerged today as the largest producer of milk in the world crossing
97 million tones annually. The organized dairy sector in India is largely dependent on
buffalo milk as they contribute about 48 percent of the total milk produced in the
country. Molecular genetic technologies will aid conventional methods of increasing
production potential of indigenous buffaloes by augmenting genetic gain in a
relatively shorter span of time. The candidate gene approach provides tools to search
for causative polymorphisms affecting quantitative traits. Signal transducers and
activators of transcription (STAT5A) and peroxisome peoliferator activated receptor
gamma cofactor 1 alpha (PPARGC1A) are transcription factor encoding genes that are
proved to be having candidate roles in milk production. The present work was carried
out to study the polymorphism in STAT5A exon 7 and exon 10 and PPARGC1A
partial exon 9 and intron 9 gene fragments by PCR-SSCP, PCR-RFLP and sequencing
in Mehsana buffaloes.
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Keywords
Animal Biotechnology, Analysis