Marker assisted consolidation of low polyphenol oxidase and rust resistance genes in high grain protein bread wheat lines
| dc.contributor.advisor | Bhagat, Indu | |
| dc.contributor.author | Gill, Manpartik S. | |
| dc.date.accessioned | 2018-09-20T04:21:17Z | |
| dc.date.available | 2018-09-20T04:21:17Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | Low polyphenol content in wheat grain is a desirable quality trait, conferring bright, amber appearance to products including chapattis. The present study is based on two donor lines (09014/23 and Clear White 515- GpC) each possessing two major recessive QTL’s for low PPO, along with rust resistance genes (i.e. Yr5, Yr15, Yr36, Lr34 and Lr37) and high grain protein gene GpC-B1. The recipient lines BWL 5189 and BWL 5190 represent versions of superior chapatti quality variety PBW 550 carrying introgression of Yr15, Yr36, Lr34 and GpC-B1. The four all possible crosses were performed at main location (2015-16) and F1’s were grown at the PAU offseason research station during summer of 2016. F2 from four crosses were raised at main season location (PAU, Ludhiana) using a strategy for early planting with the objective of obtaining two generations within the season. Seedlings germinated in propagation trays at 14-18 ̊ C were transplanted in mid October and flowered near mid December. One population of 208 plants derived from a single F1 plant from cross, 09014/23 x BWL 5189 was chosen for marker analysis. Marker PPO33, PPO29 and Wmc175 for low PPO genes i.e. PpO- A1 (on chromosome 2A), PpO- D1 (on chromosome 2D) and Yr5 (on chromosome 2B) were used in the study. The marker profiling resulted in identification of 38 plants carrying desirable alleles for three genes, either in homozygous or heterozygous condition. Out of these 38, 2 were found to be homozygous for all the three target alleles. This population along with other F2 populations were carried forward using a rapid generation advanced (RGA) strategy involving, detached tiller culture, followed by harvesting and drying of immature caryopsis with or without cold treatment. Rapid generation advance system was refined further and caryopsis harvested at 16 Days after pollination (DAP) gave desirable combination of high germination with saving of time. It was also observed that cold treatment for 2 days prior to germination was not needed. The 2 homozygous progenies had PPO score of 1.8 and 2.0 as compared to 4.2 (out of 10) in the recipient parent BWL 5189 and 1.2 for 09014/23. The protein content of these were significantly higher (13.31% and 13.14%) than the grand parental line PBW 550 (12.3%) while their grain hardness was 10.69 Kg and 12.12 Kg as compared to 12.26 Kg of PBW 550 and 8.05 of 09014/23. The PPO allele profiling was extended to a set of 87 wheat varieties to see genetic changes that had taken place at this locus as we move from landraces to modern day cultivars. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810073830 | |
| dc.keywords | Polyphenol oxidase, Quantitative trait loci, Grain protein gene B1, Bread Wheat Ludhiana, Filial | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 52 | en_US |
| dc.publisher | Punjab Agricultural University, Ludhiana | en_US |
| dc.research.problem | Marker assisted consolidation of low polyphenol oxidase and rust resistance genes in high grain protein bread wheat lines | en_US |
| dc.sub | Genetics and Plant Breeding | en_US |
| dc.subject | null | en_US |
| dc.theme | Marker assisted consolidation of low polyphenol oxidase and rust resistance genes in high grain protein bread wheat lines | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Marker assisted consolidation of low polyphenol oxidase and rust resistance genes in high grain protein bread wheat lines | en_US |
| dc.type | Thesis | en_US |