SURVIVAL RATE OF IMMATURE AND IN VITRO MATURED BOVINE OOCYTES VITRIFIED USING CRYOLOOP METHOD

Simple item page
dc.contributor.advisorMETILDA JOSEPH
dc.contributor.authorALICIAH MARIE KURIAKOSE
dc.date.accessioned2019-07-01T08:08:46Z
dc.date.available2019-07-01T08:08:46Z
dc.date.issued2016-07-05
dc.description.abstractSuccessful cryopreservation of oocyte using vitrification ensures the availability of large number of oocytes for in vitro maturation (IVM) and in vitro fertilization (IVF). The present study was carried out to evaluate effect of meiotic stage on survival rate of bovine oocytes after cryoloop vitrification and to assess viability using morphological evaluation and trypan blue staining. Ovaries of 36 slaughtered cattle were selected and randomly allotted to three groups. Oocytes were recovered by follicular aspiration. Half of the group І culture grade oocytes (n=44) were subjected to trypan blue (0.05%) staining for viability assessment and aceto-orcein staining for evaluation of nuclear status. Culture grade oocytes in group ІІ (n=38) were matured in vitro after cryoloop vitrification and culture grade oocytes in group ІІІ (n=39) were vitrified after in vitro maturation. A total of 241 oocytes were aspirated and yield of immature oocytes per cow was 6.69. Trypan blue staining of culture grade oocytes obtained by morphological evaluation in group І yielded only 81.82 per cent live oocytes. Aceto-orcein staining of group І culture grade oocytes revealed that 54.44 per cent of oocytes were in GV stage. Morphological survival rate of mature vitrified oocytes in group ІІІ was higher than immature vitrified and matured oocytes in group ІІ (66.67% vs 52.63%). Cumulus expansion, polar body extrusion and trypan blue dye exclusion rate were also higher in group ІІІ, whereas nuclear maturation rate was almost similar. The meiotic stage at which cryoloop vitrification was carried out had no significant influence on survival rate, viability, cumulus expansion, polar body extrusion and nuclear maturation rate of bovine oocytes. Hence immature bovine oocytes can be preserved using cryoloop technique, where facilities are not available for IVM. Compared to morphological evaluation, trypan blue staining technique was found to be more reliable for assessing oocyte viability.en_US
dc.identifier.citationKVASU REFERANCE CITATIONen_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810111097
dc.language.isoenen_US
dc.pages130p.en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCE-MANNUTHY,THRISSURen_US
dc.research.problemSURVIVAL RATE OF IMMATURE AND IN VITRO MATURED BOVINE OOCYTES VITRIFIED USING CRYOLOOP METHODen_US
dc.subAnimal Reproduction and Gynecologyen_US
dc.subjectnullen_US
dc.themeANIMAL REPRODUCTION, GYNAECOLOGY AND OBSTETRICSen_US
dc.these.typeM.V.Sc.en_US
dc.titleSURVIVAL RATE OF IMMATURE AND IN VITRO MATURED BOVINE OOCYTES VITRIFIED USING CRYOLOOP METHODen_US
dc.typeThesisen_US
Files
Collections
Thesis