GENE EXPRESSION AND GENOTYPIC RESPONSES TO BACTERIAL BLIGHT IN POMEGRANATE

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dc.contributor.advisorB. N. S., MURTHY
dc.contributor.authorB., TANUJA PRIYA
dc.contributor.otherS., JAGANNATH
dc.contributor.otherC., ASHWATH
dc.contributor.otherT. R., GURUPRASAD
dc.contributor.otherPATIL, PRAKASH
dc.contributor.otherC., GOPALA KRISHNAN
dc.date.accessioned2021-01-01T06:50:21Z
dc.date.available2021-01-01T06:50:21Z
dc.date.issued2016-06
dc.description.abstractThe pomegranate is cultivated since ancient times and believed to be native of Iran. It possesses various metabolites that made this crop commercially remunerative replacing subsistence farming status. However, the bacterial blight disease became a serious threat for its production in recent times. Hence, the present study was conducted at Indian Institute of Horticultural Research (IIHR), Bengaluru with a focus to identify the resistant lines and mine the genes that impart resistant reactions to facilitate breeding program. Cluster analysis classified 209 genotypes into 15 clusters with low intra-group and high intergroup similarities. Genotypes of cluster 15 possessing 108 B, 99 A, IC 318734, IIHR-30 and Daru-18 were found to confer resistance to bacterial blight incidence. The genotypes with varying degree of resistance exhibited significant variation at phenotypic and molecular level. The reactions against Xanthomonas axonopodis pv. punicae (Xap) for both highly susceptible (Ruby) and resistant (IC 318734) genotype were analyzed through physiological, biochemical and histological interventions to understand the defense mechanism. Transcriptome profiling of differentially expressed genes in resistant and susceptible genotypes through suppressive subtractive hybridization and Illumina HiSeq.TM500 (NGS) methods categorized all the genes into three groups viz., biological process, molecular function and cellular component. Subsequently, the genes identified were found confined to membrane transport, photosynthesis response, defense response, metabolic process and signal transduction sub groups. Further, genes specifically annotated to defense transcripts were catalase enzyme, transcription factors, LRR domain, trichome birefringence-like 34 and TMV resistance N-like in pomegranate with response to Xap. Thus, the investigation upshots would pave a path to identify disease resistant markers for effective crop improvement programme.en_US
dc.identifier.otherUHS13PGD73
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810158053
dc.keywordsPOMEGRANATE, T294en_US
dc.language.isoEnglishen_US
dc.pages220 ( Due to pdf. conversion there is a difference in printed & soft copy )en_US
dc.publisherUniversity of Horticultural Sciences, Bagalkot (COLLEGE OF HORTICULTURE, BENGALURU)en_US
dc.research.problemThesisen_US
dc.subHorticultureen_US
dc.themeGENE EXPRESSION AND GENOTYPIC RESPONSES TO BACTERIAL BLIGHT IN POMEGRANATEen_US
dc.these.typePh.Den_US
dc.titleGENE EXPRESSION AND GENOTYPIC RESPONSES TO BACTERIAL BLIGHT IN POMEGRANATEen_US
dc.typeThesisen_US
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