Genetic Analysis of Panthera Pardus and Panthera tigris by mtDNA PCR
| dc.contributor.advisor | Tembhurne, P. A. | |
| dc.contributor.author | Acharya, P. S. | |
| dc.date.accessioned | 2018-12-19T07:10:47Z | |
| dc.date.available | 2018-12-19T07:10:47Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | Vidarbha region, is blessed with flora & fauna where several cat families members are being residing in different forest. However, none of the report signifies the genetic analysis of these wild animals. There are always man and animal conflict reported mentioning animals being man eater or so. So the presence of particular animal is to be firmly confirmed to take any further action to avoid the killing of innocent one. Similarly, the wild animal sample collection remains one of trickiest part, however the scat sample can easily obtained by humane methods in captivity as well as in wild. With advances of genetic techniques using the scat samples to identify the species need to evaluate to enforce the protection of endangered species. Hence, the present study was undertaken to develop a rapid and cost-effective protocol for the reliable identification of tigers and leopards species from sympatric carnivore scats. The coded scat samples (24) including a confiscated scat samples nearby the leopard cells, were received from the Wildlife Research and Training Centre, Gorewada Nagpur. The samples were processed for DNA isolation using QIAamp DNA mini stool kit followed by its quantification by Nanodrop spectrophotometer. The DNA was analyzed with species specific primers targeted to mtDNA Cytochrome b gene of Panthera pardus and Panthera tigris using multiplex PCR. Furthermore, two amplicons including one confiscated scat amplicon from leopard and one amplicon from tiger were commercially sequenced. The received raw sequence data were further analyzed using different bioinformatics software tools viz., Chromas software (Version 2.1.8), clustal W, BioEdit, BLASTn and phylogenetic analysis was done using maximum likelihood by MEGA 7 software. The mtDNA analysis using the PCR showed a typical 156 bp amplicons in 19 samples of panthera pardus & 271bp amplicons for one tiger species. The homology search of mtDNA cytb gene partial sequence analysis by BLASTn also revealed our sequences are of Panthera pardus and Panthera tigris in origin as they showed homology upto 99% with respective species. The phylogenetic analysis also placed the Panthera pardus and panthera tigirs sequences in their respective species clads. From the present study, we conclude that mtDNA cytochrome b gene amplification with species specifc primers under study can be employed for the detection of Panthera pardus and Panthera tigris from scat sample successfully. Also scat sample found to useful in the monitoring of species movement, forensics tools for species identification as well in confiscated samples and in human animal conflicts cases. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810086066 | |
| dc.keywords | Animal Biotechnology; Genetic Analysis of Panthera Pardus and Panthera tigris by mtDNA PCR | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 62 | en_US |
| dc.publisher | MAFSU, Nagpur | en_US |
| dc.sub | Animal Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | Genetic Analysis of Panthera Pardus and Panthera tigris by mtDNA PCR | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | Genetic Analysis of Panthera Pardus and Panthera tigris by mtDNA PCR | en_US |
| dc.type | Thesis | en_US |