APPLICATION AND EVALUATION OF MESENCHYMAL STEM CELLS ON WOUND HEALING IN RAT

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dc.contributor.advisorPradhan, S.
dc.contributor.advisorMondal, S.
dc.contributor.advisorDas, K.
dc.contributor.advisorBag, Sadhan
dc.contributor.advisorDey, Samir
dc.contributor.authorMAHESH A, KUMARA DATTHA
dc.date.accessioned2024-02-06T14:07:58Z
dc.date.available2024-02-06T14:07:58Z
dc.date.issued2023
dc.description.abstractThe primary goal of this study is to assess the wound healing effect of mesenchymal stem cells and condition media in xenogeneic transplantation on rat skin full thickness excisional wounds. MSCs derived from bovine umbilical cord were isolated and cultured using explant and enzymatic methods. The MSC isolation technique was standardised using enzymatic digestion, as was the explant method. Because of their migratory properties, MSCs emerged from explants by day 4 and reached 70-90% confluence by day 10. MSCs were digested with collagenase (1mg/ml) for 4 hours at 370C before being plated and cultured, reaching 90% confluence after 72 hours. MSCs exhibited strong plastic adherence and fibroblast-like morphology. MSCs were cultured in vitro and differentiated into osteocytes, chondrocytes, and adipocytes according to International Society for Cell Therapy and Mesenchymal Stem Cell Committee guidelines. Xenogeneic bovine UC-MSC and bovine UC-MSC derived condition media were transplanted into two treatment groups and compared to positive, negative (vehicle), and wound healing ointment groups. A full thickness (10mm) skin punch biopsy was performed on both sides of the shoulder region, and MSC and CM were injected at the wound's periphery. Wound healing was evaluated using wound contraction (up to day 14), gross morphological evaluation (up to day 7), cytological examination of the wound bed area (up to day 7), and histo-morphological evaluation of skin samples collected on days 7, 14, 21, and 28. Daily photographs were taken from the same height, and wound closure was accelerated in the MSC and CM treated groups. Gross morphological parameters were scored, and it was discovered that all wounds in all experimental groups were grossly healthy until day 7, with enhanced granulation tissue noted in MSC and CM treated groups. Cytological analysis revealed a significant increase in mononuclear infiltration after 72-96 hours in the CM and MSC groups. Overall, histomorphological observations revealed that MSC and CM treated groups had significantly accelerated wound closure, advanced re-epithelisation and neovascularisation, mature extra-cellular matrix deposition with better collagen arrangement, and reduced scarring. Every parameter was scored and statistically evaluated.
dc.identifier.otherD-3399
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810206724
dc.language.isoEnglish
dc.pages150p.
dc.publisherWest Bengal University of Animal & Fishery Sciences, Kolkata
dc.subVeterinary Pathology
dc.themeAPPLICATION AND EVALUATION OF MESENCHYMAL STEM CELLS ON WOUND HEALING IN RAT
dc.these.typeM.V.Sc.
dc.titleAPPLICATION AND EVALUATION OF MESENCHYMAL STEM CELLS ON WOUND HEALING IN RAT
dc.typeThesis
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