“Molecular Identification and Characterization of Lumpy Skin Disease Virus (LSDV) from Cattle in Jammu”
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Date
2024-04-11
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Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu (J&K)
Abstract
The present study was undertaken for molecular identification and characterization of Lumpy Skin Disease Virus (LSDV) from cattle in Jammu district and phylogenetic analysis of LSDV. A total of 277 nasal swab samples were received from Animal Husbandary (AH) Department Jammu and were screened for the presence of LSDV by Real Time PCR (qPCR). The viral DNA was extracted by using viral nucleic acid extraction kit as per manufacturer’s protocol and LSDV P32 gene was targeted by using specific primers as recommended by OIE by using qPCR. Out of total samples tested, 99 were found to be positive for LSDV in Jammu district with occurrence of 35.74%. The breed wise occurrence of LSD was found to be highest in Sahiwal (50%) and lowest in Jersey (0%) whereas age wise occurrence of LSD was found to be highest in young animals in the age group of 0-2 years i.e., 58.33% and lowest in the age group of 6-8 years (23.40%). Higher disease occurrence was recorded in male animals i.e., 50% while only 35.63% females were found to be positive for LSD. The χ2 value was found to be non-significant for sex and breed but significant for age group affected with LSD. One of the GPCR gene amplicon was sequenced by Sanger sequencing and submitted to GENBANK under accession no. OR209679. BLAST searches were made on the sequence obtained which revealed 12bp nucleotide deletion in the GPCR gene. The phylogenetic tree was constructed by using Neighbour-Joining tree constructing method in MEGA 11 software and maximum homology was observed with sequences of Russia, Serbia, Greece and Pendik.
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