Effect of sucrose as signaling molecule on carbohydrate metabolism and antioxidant capacity in wheat (Triticum aestivum L.) under high temperature stress

Simple item page
dc.contributor.advisorBavita Asthir
dc.contributor.authorTagde, Sanjana Sanjay
dc.date.accessioned2023-12-11T12:25:53Z
dc.date.available2023-12-11T12:25:53Z
dc.date.issued2023
dc.description.abstractIn the present study, the effect of sucrose was studied on the antioxidant defense system and carbohydrate metabolism of two wheat (Triticum aestivum L.) genotypes (Unnat PBW 343 and C 306) under high temperature (HT) stress. The analysis was carried out on the seedling raised in the laboratory (25oC and 32oC) in the petri plates on germinating paper for six days. High temperature significantly decreased the activity of antioxidant enzymes such as ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) and non-enzymatic antioxidants, viz., ascorbate, glutathione reduced and glutathione oxidized content. Apparently, the activities of acid and neutral invertase, sucrose synthase (cleavage, synthesis), α- and β-amylase, and the glyoxalase enzymes (Gly I, Gly II, and Gly III) increased on the supply of exogenous sucrose in the wheat seedlings. High temperature significantly reduced shoot/root lengths, fresh weight and dry weight of both the genotypes, whereas sucrose mitigated this effect by increasing these attributes. As a defense reaction, exogenous sucrose application upregulated levels of antioxidants involved in the ascorbate-glutathione cycle in germinating seedlings under heat stress condition. Sucrose mediated amelioration of heat stress seems to be associated with the production of reactive oxygen species like H2O2, accumulation of proline which improved overall thermotolerance and increased membrane stability with the decline in the malondialdehyde content. Carbohydrate metabolism in terms of total sugars and reducing sugars increased under both control and stress condition. High temperature accentuated sucrolytic enzymes viz. invertases and sucrose synthase. Likewise, a significant increase in the glyoxalase (Gly I, Gly II and Gly III) activity along with methyl glyoxal (MG) content was recorded. Higher build up of sugars and starch particularly in the shoot of Unnat PBW 343 indicates that sugars play a very important role in heat stress tolerance as it reduces oxidative stress by decreasing the content of H2O2 and increasing antioxidants. Growth related parameters were significantly improved with sucrose under HT stress. Hence, the interplay of several biochemical parameters including osmolyte may be involved in improving seed germination under stress conditions. Thus, sucrose could be used as an effective signaling molecule for improving seedling growth under stress condition.
dc.identifier.citationTagde, Sanjana Sanjay (2023). Effect of sucrose as signaling molecule on carbohydrate metabolism and antioxidant capacity in wheat (Triticum aestivum L.) under high temperature stress (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810202518
dc.keywordsAntioxidant defense system
dc.keywordscarbohydrate metabolism
dc.keywordshigh temperature stress
dc.keywordssucrose signaling
dc.keywordsTriticum aestivum
dc.keywordswheat
dc.language.isoEnglish
dc.pages68
dc.publisherPunjab Agricultural University
dc.research.problemEffect of sucrose as signaling molecule on carbohydrate metabolism and antioxidant capacity in wheat (Triticum aestivum L.) under high temperature stress
dc.subBiochemistry
dc.themeEffect of sucrose as signaling molecule on carbohydrate metabolism and antioxidant capacity in wheat (Triticum aestivum L.) under high temperature stress
dc.these.typeM.Sc
dc.titleEffect of sucrose as signaling molecule on carbohydrate metabolism and antioxidant capacity in wheat (Triticum aestivum L.) under high temperature stress
dc.typeThesis
Files
Collections
Thesis