Adaptation of cotton shoot apex culture to agrobacterium mediated transformation (Gossypium Spp)
| dc.contributor.advisor | Bharose, A. A. | |
| dc.contributor.author | Rehana Mirza Sajeed Baig | |
| dc.date.accessioned | 2018-06-30T06:37:33Z | |
| dc.date.available | 2018-06-30T06:37:33Z | |
| dc.date.issued | 2013-08-19 | |
| dc.description.abstract | Cotton (Gossypium spp.) is an excellent natural source of textile fibre. It is a crop of significant value throughout the world because it is not only a source of natural fibre, but also an oilseed crop. Because of its high economic importance, considerable attention has been paid to improving cotton plants against biotic and abiotic stress through transgenic technology. The present investigation was carried out with an aim to study fire regeneration potential and standardize the transformation protocol for cotton genotypes NH-615 and NH-635, which are the straight cultivars ,so that the farmers can conserve the germplasm and get seeds available at low cost. The results of regeneration were statistically analysed using FRBD under three replications. And the results of transformation were analysed using RBD under four replications. The shoot apex explants were used for regeneration of cotton. The explants (shoot apices) showed highest response for multiple shoot induction on MS media supplemented with BAP and NAA. Similarly the elongation of shoots were reported on MS media supplemented with GA3 and BAP, also the media MS with DBA was found significant for induction of roots to the growing explants. Simultaneously an Agrobacterium mediated transformation protocol was optimized in the same cultivar NH-615 using the disarmed Agrobacterium strain EHA-105 harboring binary vector pCAMBIA, carrying CrylAc gene. The seedlings with just emerging plumule were infected with a separate Agrobacterium strain carrying the gene construct pBI-121 made up from engineered reporter gene, p-glucuronidase (Gus) and a selectable marker gene neomycin phosphotransferasell to confirm the transformability of the cultivar. Gus histochemical analysis of the young leaves and hypocotyledonary node revealed that the gene was successfully integrated into cotton genome and expressed in the transgenic plants. Through statistical analysis is proved that NH-615 has better results for transformation and regeneration than NH-635 using the shoot apices as explantf | en_US |
| dc.identifier.other | T06846 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810056001 | |
| dc.keywords | Cotlyledons, Epicotyl, Antibiotics, Agrobacterium, Colonization | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 84 | en_US |
| dc.publisher | Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani | en_US |
| dc.sub | Agricultural Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | AGRICULTURE | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Adaptation of cotton shoot apex culture to agrobacterium mediated transformation (Gossypium Spp) | en_US |
| dc.type | Thesis | en_US |