SERO-PREVALENCE, MOLECULAR DETECTION AND ASSESSMENT OF FARM LEVEL RISK FACTORS OF JAPANESE ENCEPHALITIS VIRUS INFECTION IN PIGS OF LAKHIMPUR DISTRICT OF ASSAM
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Date
2016-07
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College of Veterinary Science, Assam Agricultural University Khanapara, Guwahati-781022
Abstract
An investigation was carried out in the Department of Veterinary Public Health, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-22, to study the Sero-prevalence, molecular detection and assessment of farm level risk factors of Japanese encephalitis virus infection in pigs of Lakhimpur district of Assam. The samples were collected from 145 pig farms both organized and unorganized in 9 blocks of Lakhimpur district with a total 58 numbers of samples per block of the district, during the year 2015-16 . Out of 522 serum samples collected, 20 (3.83%) were found to be sero-positive by Indirect ELISA for Japanese encephalitis virus infection. Among the 9 blocks the highest prevalence was found in Boginadi block (8.62%) followed by North Lakhimpur (6.90%), Nowbachia (6.90%), Dhokuakhana (5.17%), Bihpuria (3.45%), and Karunabari (3.45%). No prevalence was found in Telahi and Narayanpur blocks (0%). Age wise higher prevalence (4.46%) was recorded in the age group above 12 months, sex wise in female (4.41%) pigs and breed wise slightly higher prevalence was observed in local pigs (4.11%). however there was no significant association in sero-prevalence of Japanese encephalitis with respect to age , sex , and breed of pigs. All the samples subjected for RT-PCR were found to be negative for E gene of JEV by RT-PCR. Assessment of the farm level risk factors included in the present study viz. closeness to rice fields, standing water sources, exposure of wild bird to pig farms, mosquito exposure to pig sheds showed that all the risk factors were significantly associated with sero-positiviy of JE in pig farms.
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