ISOLATION, IDENTIFICATION, MOLECULAR CHARACTERIZATION AND DETECTION OF VIRULENCE ASSOCIATED GENES OF PASTEURELLA MULTOCIDA OF AVIAN ORIGIN
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Date
2013
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Publisher
Anand Agricultural University, Anand
Abstract
The present study was undertaken with a view of isolation and identification of
P. multocida from suspected cases of fowl cholera in different avian species.
A total of 312 samples were collected from suspected cases, of which 24
(7.69%) P. multocida isolates (22 from emu and 2 from chicken) were isolated using
blood agar as primary culture medium. These isolates were studied for their
biochemical behaviour, in vitro antibiotic sensitivity pattern, P. multocida species
specific PCR (PM-PCR), molecular characterization by multiplex PCR assay for
capsular typing and detection of various virulence associated genes by PCR.
Description
Keywords
Veterinary Microbiology, Study