Isolation and screening of pesticide-tolerant phosphate-solublizing bacteria from potato rhizosphere
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Date
2021
Authors
Manpreet Kaur
Journal Title
Journal ISSN
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Publisher
Punjab Agricultural University, Ludhiana
Abstract
The present study was carried out with the aim to develop pesticide-tolerant phosphatesolubilizing
bacterial inoculants for improving the growth and phosphorus uptake in potato. A
total of 37 phosphate-solubilizing bacteria were isolated from the rhizosphere of potato
growing at different locations of Punjab and Himachal Pradesh. The bacterial isolates showed
phosphate solubilization index ranging from 1.3 to 6.7 mm on modified Pikovskaya agar and
phosphate solubilization in liquid medium varying from 115.4 – 919 µg ml-1 with significant
difference among the isolates. The isolates were further tested for their tolerance against two
commonly used pesticides in potato on minimal salt agar medium amended with three
concentrations 1.25, 2.5 and 7.5 μl ml-1 (lower, recommended and higher dosages) of
chlorpyrifos and 0.187, 0.375 and 0.75 g ml-1 (lower, recommended and higher dosages) of
carbofuran. Out of 37 PSB isolates, only 7 showed pesticide tolerance. The phosphate
solubilising ability of the isolates was found to decrease significantly with increased
concentrations of pesticides. Seven pesticide-tolerant PSB were identified as Arthrobacter
oxydans (PR2PSB2), Bacillus licheniformis (PR3PSB9, PR3PSB10, PR3PSB12 and PR3PSB13),
Bacillus subtilis (PR3PSB11) and Bacillus flexus (PR4PSB1) based on phenotypic features,
biochemical tests and Matrix-Assisted Laser Desorption/Ionization time of Flight Mass
Spectroscopy (MALDI-TOF-MS) analysis. Four pesticide-tolerant and phosphate-solubilizing
bacterial isolates belonging to different species were tested for their ability to promote growth
and phosphorus uptake in potato in pesticide amended soils under greenhouse conditions in
pots. A significant difference was observed in the growth parameters, P content in shoots and
soil P with the use of pesticide-tolerant PSB strains over the uninoculated control. The plant
growth promotion and phosphorus content decreased with an increase in pesticide
concentrations. The bacterial strains also differed significantly in their ability to promote the
potato growth and phosphorus uptake. The highest growth promotion and P uptake was
observed with consortium 1 containing Bacillus licheniformis, Bacillus flexus and Bacillus
subtilis (PR3PSB10, PR4PSB1 and PR3PSB11). The study has led to the selection of pesticidetolerant
phosphate-solubilizing bacterial consortium for improved growth and phosphorus
uptake in potato.
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Citation
Manpreet Kaur (2021). Isolation and screening of pesticide-tolerant phosphate-solublizing bacteria from potato rhizosphere (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.