SUPEROVULATORY RESPONSE, EMBRYO COLLECTION AND TRANSFER IN CROSSBRED COWS

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Date
1996
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES Mannuthy - Thrissur
Abstract
Out of eleven crossbred cows superovulated with 2000 lu of PMSG on day eleven and 25 mg pgp, alpha 48 h later, nine animals ,81.8t, exhibited oestrum after an average interval of 39.44 ± 2.44 h. Average duration of oestrum was 40.00 ± 2.00 h with 66.7 per cent of them exhibiting intense heat signs and 33.3 per cent exhibiting only moderate heat signs. Average number of corpora lutea and unovulated follicles in both the ovarres put together was 6.67 r 0.50 and 6.22 s o 57 respectively, ovarian response was more in right ovary than in left ovary, though not statistically significant. Average fluid recovery on flushing was 79.5 per cent which was comparatively low. The average e^ryo recovery and percentage of embryo recovery were 3.38 ^ 0.70 and 53.11 , 10.18 respectively, which was comparatively lower. The reason for poor recovery of embryo was attributed to poor fluid ery on flushing. The average number and percentage of fransferrable embryo recovered were 1.50 i 0.53 and 33 34 i 10^14. Reason for these lower rates were attributed to loss of ryo quality, due to prolonged action of PMSG. Donors of parity over three performed better on =, us i.ng, than those below three. Animals o®f^PSi^ovulation and -animals of age group six to nine years produced more transf err;,hi o k ransferrable embryos than cows of age group ten and above. A conception rate of 30 per cent was achieved after ^^^^sfer of enibryos to naturally synchronised recipients. Heifers appeared to be better recipients than cows. Although a high incidence of abortion was encountered, birth of an embryo transfer male calf was also recorded in the study. All the donor cows came into regular oestrous cycle within four months of superovulation treatment.
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