HISTOMORPHOLOGICAL AND HISTOCHEMICAL STUDY OF FALLOPIAN TUBE, UTERUS, CERVIX AND VAGINA OF THE SURTI BUFFALO (Bubalus bubalis ) DURING VARIOUS PHASES OF ESTROUS CYCLE
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Date
1990
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AAU, Anand
Abstract
The present investigation on histomorphological and histochemical changes in fallopian tube, uterus, cervix and vagina during various phases of estrous cycle, was carried out on 24 (6 in each phase) non-gravid genitalia of Surti buffaloes. The samples for the study, were collected from infundibular, ampullary and isthmic regions of the fallopian tube, the middle part of uterine horn, the external os of the cervix and from the mid-vaginal region and were preserved i n 10 per cent neutral buffered formalin. Then they were processed to obtain paraffin sections and were stained with Harris' haematoxylin and eosin. The histochemical study was carried out on the frozen sections, taken immediately after collection of samples, for detection of mucopolysaccharides. Alkaline phosphatase (AKP) and succinic dehydrogenase (SDH) activity. The epithelium of fallopian tube was pseudostratified low to tall columnar with varying member of cilia and goblet cells in different region and phases. The uterine epithelium was medium to low columnar with patches of pseudostratified columnar epithelium during estrus phase, while other phases showed pseudostratified tall columnar epithelium with goblet cells. The epithelium of uterine gland was %all columnar in type, but it was low columnar with patches of pseudostratified columnar epithelium in superficial uterine glands during estrus and metestrus phases. The simple columnar cervical epithelium was tall during proestrus and estrus phases and low during metestrus and diestrus phases. The vaginal epithelium was stratified squamous, non-keratinized but the epithelial type of superficial layer and total number of layer varied during different phases. The thickness of epithelium in different organs varied during various phases. The thickness was maximum during estrus phase in fallopian tube, superficial as well as basal uterine glands, cervix and vagina. However, the thickness of endometrium and its epithelium, was maximum during diestrtis phase. The number of primary folds, was maximum during diestrus phase while, that of secondary folds was maximum in estrus phase in all the three regions of fallopian tube. Amongst the three regions of fallopian tube, the primary and secondary folds were decreasing in number from infundibulum to isthmus. The lumen diameter was maximum in infundibulum and minimum in isthmus and it also showed phase-wise differences.
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ANATOMY, A STUDY