IDENTIFICATION AND CLASSIFICATION OF VARIOUS OYVMV (OKRA YELLOW VEIN MOSAIC VIRUS) ISOLATES FROM DIFFERENT AGROCLIMATIC ZONES OF GUJARAT USING NEXT GENERATION SEQUENCING

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dc.contributor.advisorDr. Akarsh Parihar
dc.contributor.authorSOLANKI GAUTAMKUMAR VELJIBHAI
dc.date.accessioned2021-06-09T04:01:41Z
dc.date.available2021-06-09T04:01:41Z
dc.date.issued2019
dc.description.abstractThe present investigation entitled “Identification and classification of various OYVMV (Okra Yellow Vein Mosaic Virus) isolates from different agro-climatic zones of Gujarat using Next Generation Sequencing” was carried out at the Department of Agricultural Biotechnology, Anand Agricultural University, Anand during 2017-19. During June-November 2018, total sixteen samples of OYVMV infected okra leaves were collected from eight different agro-climatic zones of Gujarat having okra under cultivation. Viral DNA isolation from Yellow Vein Mosaic Virus infected okra leaf samples was carried out by Extract-n-Amp Plant PCR kit. After isolation of viral genomic DNA from sixteen samples, gel electrophoresis was carried out using 0.8% agarose gel. The DNA bands corresponding to viral genome from the 16 different viral DNA samples run on the agarose gel were eluted and purification through promega purification kit. the ssDNA was quantified by NanoDrop® ND-1000. The Navsari samples gave the highest quantity of viral genomic ssDNA (44.7 ng/μl) followed by Mehsana (43.3 ng/μl). The ssDNA was converted into dsDNA by using cDNA synthesis kit. After purification, the Qubit® 3.0 Fluorometer was used to quantify the viral genomic dsDNA and the highest quantity was recorded by sample from Kheda (38.3 ng/μl) followed by Navsari (29.2 ng/μl).en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810169773
dc.keywordsOYVMV (OKRA YELLOW VEIN MOSAIC VIRUS), ISOLATES, AGROCLIMATIC, ZONES, GUJARAT, NEXT GENERATION SEQUENCINGen_US
dc.language.isoEnglishen_US
dc.publisherDEPARTMENT OF AGRICULTURAL BIOTECHNOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANANDen_US
dc.subBiotechnology and molecular Biologyen_US
dc.themeIDENTIFICATION AND CLASSIFICATION OF VARIOUS OYVMV (OKRA YELLOW VEIN MOSAIC VIRUS) ISOLATES FROM DIFFERENT AGROCLIMATIC ZONES OF GUJARAT USING NEXT GENERATION SEQUENCINGen_US
dc.these.typeM.Scen_US
dc.titleIDENTIFICATION AND CLASSIFICATION OF VARIOUS OYVMV (OKRA YELLOW VEIN MOSAIC VIRUS) ISOLATES FROM DIFFERENT AGROCLIMATIC ZONES OF GUJARAT USING NEXT GENERATION SEQUENCINGen_US
dc.typeThesisen_US
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