EPIDEMIOLOGY OF CERTAIN BACTERIAL AND VIRAL DISEASES CAUSING NEONATAL MORTALITYIN PUPS
Loading...
Date
2004
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
Abstract
The present study was conducted to assess the role of bacterial and certain
viral etiology, to conduct antibiogram and to estimate the nonspecific factors
associated with neonatal mortality in pups.The bacterial isolates from milk of
bitches were identified as Staphylococcus aureus (29 per cent) Staphylococcus
intermedius (six per cent), Klebsiella pneumoniae (47 per cent), Proteus vulgaris (12
per cent) and Pseudomonas aeruginosa (six per cent). Antibiogram of the isolates
showed that majority of the isolates from milk were sensitive to ciprofloxacin (71
per cent), followed by amoxycillin (65per cent), gentamicin (41per cent),
enrofloxacin (59 per cent), norfloxacin (18 per cent) chloramphenicol (12 per cent)
and amoxy-clav (6 per cent).Blood samples taken from two bitches yielded bacterial
growth, which were identified as Staphylococcus aureus (50 per cent) and Klebsiella
pneumoniae (50 per cent). The bacterial isolates from blood were lOOper cent
sensitive to ciprofloxacin and chloramphenicol, 50 per cent to gentamicin, 50 per
cent to amoxycillin and resistant to enrofloxacin and norfloxacin. The bacteria
isolated from the vaginal samples were identified as Hafnia alvei (Enterobacter
a/ve/)(six per cent), Klebsiella pneumoniae (35 per cent), Staphylococcus aureus (12
per cent), Escherichia coli (12 per cent), Proteus vulgaris (23 per cent),
Pseudomonas aeruginosa (six per cent) and Staphylococcus intermedius (six per
cent). The vaginal isolates were sensitive to ciprofloxacin (88 per cent), gentamicin
(53 per cent), amoxycillin (59 per cent), enrofloxacin (71 per cent), chloramphenicol
(59 per cent) and norfloxacin (35 per cent).
None of the milk, blood or vaginal swabs taken from the control group
yielded any bacterial growth. Isolates were obtained from heart blood and from liver
and lung of pups. These included Proteus vulgaris{20 per cent),Pseudomonas
aeruginosa{40 per cent) and Klebsiella pneumoniae{40 per cent). Fourty per cent of
the bacterial isolates from pups were sensitive to gentamicin and chloramphenicol,
80 per cent to ciprofloxacin, 60 per cent to amoxycillin and 20 per cent to
norfloxacin and enrofloxacin.
The role of parvovirus as a causative agent for neonatal mortality was
studied by subjecting the liver, lung,intestine spleen and kidney tissue of dead pups
to AGID and HA for detection of parvoviral antigen and one sample was positive by
HA. This was confirmed by haemagglutination inhibition test. None of the serum
from the bitches gave positive result for antibodies against parvoviral antigen in agar
gel immunodiffusion test.
The titre of antibodies of bitches were assessed by HI. HI titre of the
vaccinated bitches ranged from eight to 64.Only one bitch was having the protective
titre. All the unvaccinated bitches were not having any titre of antibodies against
parvo by HI test
Description
Keywords
null
Citation
KVASU REFERENCE CITATION