EVALUATION OF OUTER MEMBRANE PROTEIN BASED IgM DOT ENZYME LINKED IMMUNOSORBENT ASSAY FOR THE RAPID DIAGNOSIS OF CANINE LEPTOSPIROSIS

Simple item page
dc.contributor.advisorSiju Joseph
dc.contributor.authorG. ABHINAY
dc.date.accessioned2020-07-10T05:58:11Z
dc.date.available2020-07-10T05:58:11Z
dc.date.issued2011
dc.description.abstractLeptospirosis is an important public health threat in many places of south India, including Kerala. Diagnosis of leptospirosis is mostly dependent on serological methods. The undercurrent antibodies caused due to prior infection or vaccination, complicate the confirmation of disease, especially in an endemic region. Therefore, tests based on detecting leptospiral IgM antibodies can serve as a useful approach in diagnosing acute canine leptospirosis. The potential of IgM dot ELISA for diagnosis of acute canine leptospirosis was evaluated in this study. A total of 114 canine serum samples collected from clinical cases during the period of September, 2009 to January, 2011, presented at University Veterinary Hospitals at Mannuthy and Kokkalai, Thrissur were employed in the study. The samples included those collected from 22 healthy vaccinated animals and 28 healthy unvaccinated animals. The rest of the samples were taken from cases suspected for acute leptospirosis. Among 114 samples screened with MAT, 86 were positive cases, out of which 81 gave positive reaction with LAT. Among the 28 cases shown as negative by MAT, 23 showed a negative reaction with LAT. Among 81 cases diagnosed as positive by both, MAT and LAT, 13 samples showed positive results with IgM dot ELISA, showing a prevalence of 16.04 per cent. The serum samples from the healthy vaccinated animals and the healthy unvaccinated animals were shown as negative, when tested with IgM dot ELISA, proving the specificity of the test. Convalescent sera, collected from 8 cases among the 13 positive reactors of IgM dot ELISA when screened with MAT showed a four-fold increase in MAT titers, proving the sensitivity of the test. Latex agglutination test used in the study could prove useful as a convenient screening test for leptospirosis, under field conditions. However, LAT failed to differentiate between acute and chronic carriers of the infection. The OMP based IgM dot ELISA reported in the study would thus be of great value in diagnosing acute cases of canine leptospirosis. The test being rapid and economical, not requiring any special equipment or technical expertise, makes it suitable as a field level test.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810148950
dc.keywordsEVALUATION OUTER MEMBRANE PROTEIN BASED IgM DOT ENZYME LINKED IMMUNOSORBENT ASSAY FOR RAPID DIAGNOSIS CANINE LEPTOSPIROSISen_US
dc.language.isoenen_US
dc.pages56en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSURen_US
dc.research.problemEVALUATION OF OUTER MEMBRANE PROTEIN BASED IgM DOT ENZYME LINKED IMMUNOSORBENT ASSAY FOR THE RAPID DIAGNOSIS OF CANINE LEPTOSPIROSISen_US
dc.subVeterinary Microbiologyen_US
dc.subjectnullen_US
dc.themeEVALUATION OF OUTER MEMBRANE PROTEIN BASED IgM DOT ENZYME LINKED IMMUNOSORBENT ASSAY FOR THE RAPID DIAGNOSIS OF CANINE LEPTOSPIROSISen_US
dc.these.typeM.V.Sc.en_US
dc.titleEVALUATION OF OUTER MEMBRANE PROTEIN BASED IgM DOT ENZYME LINKED IMMUNOSORBENT ASSAY FOR THE RAPID DIAGNOSIS OF CANINE LEPTOSPIROSISen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
G.ABHINAY(2009-13-143).pdf
Size:
1.02 MB
Format:
Adobe Portable Document Format
Description:
EVALUATION OF OUTER MEMBRANE PROTEIN BASED IgM DOT ENZYME LINKED IMMUNOSORBENT ASSAY FOR THE RAPID DIAGNOSIS OF CANINE LEPTOSPIROSIS
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Thesis