Isolation and screening of efficient ligninolytic fungi
| dc.contributor.advisor | Goyal, Sneh | |
| dc.contributor.author | Bhatia, Tanvi | |
| dc.date.accessioned | 2016-09-09T09:09:04Z | |
| dc.date.available | 2016-09-09T09:09:04Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | Lignin, the most abundant aromatic biopolymer on earth, is extremely recalcitrant to degradation. By linking to both cellulose and hemicellulose, it creates a barrier to any chemicals or enzymes and prevents the penetration of lignocellulolytic enz ymes into the interior lingocellulosic structure. Lignin is the major hurdle in the degradation of lignocellulosic biomass. It is degraded by an enzyme complex containing three enzymes namely laccase, manganese peroxidase and lignin peroxidase that are collectively known as ligninases. These enzymes are produced by several microorganisms, commonly by fungi but most of them have high production cost and no efficiency in enzyme production and its enzyme activit y. Therefore in the present investigation, a total of 24 mutually distinct fungi were isolated from different ecological niches such as leaf and litter waste, garbage dumpin g site, mushr oom waste, compost, ver micompost, paper and pulp waste and biogas slurry. Out of these 24 fungal isolates, 16 fungal cultures were screened as ligninolytic fungi by observing the formation of zone of clearance on malt extract agar plates containing aniline blue dye and tannic acid. The zone of clearance ranged from 1.03 to 1.20 Isolate HST15 formed the largest zone of clearance of 1.20. The fungal isolate, HST9 was showing highest laccase (15.5 U/ml) and Manganese peroxidase (4 U/ml) activity among the isolates while maximum lignin peroxidase activity was observed in HST15 (21U/ml). On the basis of lignin peroxidase activity, HST15 was selected as the best ligninolytic fungal isolate. The selected fungal isolates were morphologically identified on the basis of their hyphae, sporangiophore and spores. Among the five best fungal isolates, three were identified to be ascomycetes and two were basidiomycetes. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/76184 | |
| dc.language.iso | en | en_US |
| dc.publisher | CCSHAU | en_US |
| dc.sub | Microbiology | |
| dc.subject | Lignin, Fungal isolate, Ligninolytic enzyme, Ligninases, Laccase, Manganese peroxidase, Lignin peroxidase | en_US |
| dc.these.type | M.Sc | |
| dc.title | Isolation and screening of efficient ligninolytic fungi | en_US |
| dc.type | Thesis | en_US |
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