EXPRESSION OF CERTAIN CYTOKINES IN RELATION TO PERSISTENCE OF FOOT AND MOUTH DISEASE VIRUS TYPE ‘O’ IN CATTLE
| dc.contributor.advisor | Sharma., K. | |
| dc.contributor.author | Baro, Sangeeta | |
| dc.date.accessioned | 2019-07-31T11:30:01Z | |
| dc.date.available | 2019-07-31T11:30:01Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | The present study was undertaken to detect foot-and-mouth disease virus serotype ‘O’ in oro-pharyngeal fluid (OP fluid) and to quantify cytokines IL-1α, IL- 1β, IFN-α, TNF-α in blood of recovered animal by Real time PCR. Typing of infected clinical samples suspected for FMDV was done by sandwich ELISA followed by simultaneous detection of serotype by multiplex-PCR and detection of antibodies against NSP was done by DIVA ELISA in serum. The Relative Quantification (RQ) values for IL-1α gene during outbreak was 1.383 ±0.405 and after one month of post infection the RQ value was found to be 2.0223 ±0.592 which was found to be upregulated. Subsequently after three month of post infection the expression level of IL-1α was 23.8788±.993 which was upregulated. Later the expression level of IL-1α at 6month and nine month were 1.0223±0.299 and 1.9899±0.565 respectively. IL-1β gene expression was studied and the RQ values was found to be 0.0097±0.002 during one month of post infection which is down regulated and subsequently become undetectable during 3 month and in subsequent period of study period. The expression of IL-1β down regulation was observed in month 1 of post infection, whereas in subsequent period of the study the IL-1β was undetectable. Expression of IFN-α gene during outbreak was 1.0131±0.296. Up egulation of IFN-α in the 15 animals were found during 1, 3, 6 and 9 month respectively. The mRNA expression of TNF- α was studied and found to be upregulated during outbreak and during 1, 3, 6 and 9 month and the level of expression was 1.2361±0.362, 1.6346±0.478, 3.0521±0.893, 2.1447±0.628 and 1.3484±0.394 respectively. The present study thus supports the notion that real-time PCR is a powerful technique for reliable detection of persistent FMDV in recovered animals. The findings also indicated that IL-1α, IFN-α and TNF-α genes were gradually upregulated upto 3 months but IL-1β found to be down regulated with progression of recovery of the animals from the disease. Down regulation of the genes may be due to subside of the acute infection. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810118144 | |
| dc.keywords | CYTOKINES, FOOT AND MOUTH DISEASE, VIRUS TYPE ‘O’ , CATTLE | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 125p. | en_US |
| dc.publisher | College of Veterinary Science Assam Agricultural University Khanapara, Guwahati-781022 | en_US |
| dc.sub | Veterinary Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | EXPRESSION OF CERTAIN CYTOKINES IN RELATION TO PERSISTENCE OF FOOT AND MOUTH DISEASE VIRUS TYPE ‘O’ IN CATTLE | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | EXPRESSION OF CERTAIN CYTOKINES IN RELATION TO PERSISTENCE OF FOOT AND MOUTH DISEASE VIRUS TYPE ‘O’ IN CATTLE | en_US |
| dc.type | Thesis | en_US |