ASSOCIATION OF BUTYROPHILIN GENE POLYMORPHISMS WITH MILK YIELD AND FAT PER CENT IN CROSSBRED CATTLE OF KERALA

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dc.contributor.advisorLali F. Anand
dc.contributor.authorPOTU HEMANTH
dc.date.accessioned2022-10-29T09:35:19Z
dc.date.available2022-10-29T09:35:19Z
dc.date.issued2021-09-29
dc.descriptionSubmitted in partial fulfilment of the requirement for the degree of Master of Veterinary Science of Animal Genetics and Breedingen_US
dc.description.abstractGenetic variability of butyrophilin (BTN1A1) gene among 151 crossbred cattle of Kerala was investigated and a total of 17 single nucleotide polymorphisms (SNPs) were detected while 13 being novel. Among the SNPs detected three polymorphisms G21A, A465G and G506A were selected for detailed analysis. The study population was genotyped using high resolution melt (HRM) curve analysis and two genotypes GG (0.86) and GA (0.14) were revealed for G21A polymorphism, while KK (0.25) and KL (0.75) for A465G transition and MM (0.25) and MN (0.75) with respect to G506A polymorphism. Chi-square analysis revealed the presence of population in H-W equilibrium only with respect to SNP G21A. General linear model-univariate analysis was carried out to study the association of SNPs with milk production traits by considering herd, season of calving and parity as non-genetic factors and trait as a dependent variable. Association analysis of G21A revealed significantly higher 305- day milk yield (GG: 2720.74 ± 122.92 kg; GA: 3250.20 ± 183.24 kg), fat yield (GG: 106.55 ± 4.32 kg; GA: 126.30 ± 13.35 kg) and SNF yield (GG: 211.52 ± 9.20 kg; GA: 246.90 ± 13.70 kg) for GA genotype (P≤0.05) and SNF per cent (GG: 7.80 ± 0.04 %; GA: 7.65 ± 0.07 %) for GG genotype. Similarly, MN genotype of G506A had shown significantly higher (P≤0.05) 305-day milk yield (MM: 2358.06 ± 141.46 kg; MN: 3097.51 ± 121.92 kg), fat yield (MM: 94.14 ± 5.00 kg; MN: 119.95 ± 4.31 kg), SNF yield (MM: 183.14 ± 10.50 kg; MN: 239.08 ± 9.03 kg) and MM genotype for fat per cent (MM: 4.10 ± 0.08 %; MN: 3.90 ± 0.70 %). However, KK genotype of A465G had shown a significantly higher (P≤0.01) fat per cent (KK: 4.14 ± 0.08 %; KL: 3.91 ± 0.07 %) and SNF per cent (KK: 7.86 ± 0.06 %; KL: 7.73 ± 0.05 %). The current study recommends alleles G, K and M as favourable with respect to SNPs G21A, A465G and G506A, respectively for breeding programmes in crossbred cattle of Kerala.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810189448
dc.keywordsBUTYROPHILIN GENE, Butyrophilin ,Butyrophilin protein, Functional role of butyrophilin ,Polymorphic studies in BTN1A1 gene, Association studies in BTN1A1 gene, BTN1A1 gene , Isolation of DNA from blood, Detection of SNPs in BTN1A1 gene , Determination of concentration, purity and quality of DNA , Genotyping of crossbred cattle using HRM analysis, Milk sample collection , Milk production traits, Gerber’s centrifugation method, Estimation of test day SNF per cent using automatic milk analyser , Amplification of BTN1A1 gene ,Determination of concentration purity and quality of DNA,Detection of SNPs in BTN1A1 gene,Genotyping of SNP G21A,Genotyping of SNP A465G ,Genotype and allele frequencies ,Single nucleotide polymorphism analysis,en_US
dc.language.isoEnglishen_US
dc.pages132 p.en_US
dc.publisherKerala Veterinary and Animal Sciences University, Pookodeen_US
dc.research.problemDetection of single nucleotide polymorphisms (SNPs) in exon 1, 3 and 8 of Butyrophilin gene (BTN1A1) in crossbred cattle of Kerala, Genotyping of crossbred cattle with respect to SNPs detected, Association study of identified SNPs with milk yield and fat per cent.en_US
dc.subAnimal Genetics and Breedingen_US
dc.themeBUTYROPHILIN GENE POLYMORPHISMS WITH MILK YIELD AND FAT PER CENT IN CROSSBRED CATTLEen_US
dc.these.typeM.V.Sc.en_US
dc.titleASSOCIATION OF BUTYROPHILIN GENE POLYMORPHISMS WITH MILK YIELD AND FAT PER CENT IN CROSSBRED CATTLE OF KERALAen_US
dc.typeThesisen_US
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