MOLECULAR CHARACTERIZATION AND DIVERSITY OF ENTOMOPATHOGENIC NEMATODES IN NORTH BIHAR
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Date
2022
Authors
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Journal ISSN
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Publisher
Dr.RPCAU, Pusa
Abstract
A random survey was conducted, between November, 2021 to April, 2022 to
isolate the population of entomopathogenic nematodes. A total of 100 soil samples
were gathered from diverse habitats, including the Dholi campus and the village of
Muraul in Bihar. Entomophilic nematodes were found in five positive samples. The
total frequency of occurrence of 5 %, recorded with Dholi having the highest
frequency (10 %) and Muraul village with lowest incidence (0 %). The species
identification was verified by morphological and molecular characterization. The ITS
region of rDNA was used to characterise the molecules. The DNA sequences were
submitted to GenBank, and an accession number was received. The nematode was
identified as Acrobeloides spp. using phylogenetic and molecular analysis. The scope
of research in this area will open avenues and revealation about these nematode
species to suffice both the regions' population and the research.