Molecular characterization of basmati introgression lines for spikelet per panicle QTL (qSPP2.1) AND transfer of neck blast resistance in basmati rice
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
The present day Basmati rice cultivars have lower yield potential particularly due to lower spikelet per panicle. In this study, Basmati introgression lines (BasILs) have been used which were developed by backcrossing of Punjab Basmati 3 as recurrent parent with O. longistaminata derived introgression line LIL-427 having significantly higher spikelet per panicle QTL (qSPP2.1) as donor parent. A total of 115 BasILs in BC1F5 and BC2F4 generations were evaluated in replicated trial for yield and quality traits during the kharif season 2019 at the experimental area, School of Agricultural Biotechnology, PAU, Ludhiana. Data was taken from five randomly selected plants for all the traits except plot yield and
averaged. Range of variation was observed among BasILs for yield and quality traits viz; plant height, number of tillers per plant, spikelet per panicle, spikelet percentage, 1000 grain weight, grain yield, milled rice recovery (MRR), head rice recovery (HRR), grain length (GL), grain breadth (GB), length breadth ratio (L/B) and kernel length after cooking (KLAC). Analysis of variance revealed significant variation for all the traits except KLAC. A number of BasILs were identified having desirable variation for combination of different yield and quality traits. Mean spikelet per panicle in BasILs ranged from 26.7-195.4 as compared to recurrent parent Punjab Basmati 3 (81.0). Based on this variation, 46 BasILs
containing 23 BasILs with significantly higher and 23 with significantly lower spikelet per panicle were selected to know the proportion of parental genome contribution with the help of 117 polymorphic SSR markers. Chromosome-wise graphical genotypes for each individual were prepared using GGT v 2.0 software which represented a clear-cut distinction between recurrent and donor parent genome per chromosome. The proportion of the recurrent parent genome ranged from 21.0-54.7 % with an average of 40.1 % for all BasILs. The proportion of the donor genome for the BC2F4 and BC1F5 progenies ranging from 37.4-75.2 % with an average of 52.5 %. The Punjab Basmati-3 accounted for an average introgression of 40.1 % in BC1F5 and 67.7 % at BC2F4 progenies. The estimated average of LIL-427 genome was 52.5 % in BC1F5 and 21.8 % in BC2F4. The 46 BasILs were further analyzed with Punjab Basmati 3 gene specific markers viz. aroma (Badh 1 & Badh 2), bacterial blight resistance (xa13 & Xa21) and amylose (RM 190). Of these, six lines were found to be positive for all the genes. Since neck blast caused by Magnaporthe oryzae remains to be the major production constraint, therefore, another experiment was undertaken with the objective to incorporate two major QTL namely qBL3 and qBL7 in the genetic background of Pusa Basmati 1121. Based on evaluation of 69 BC1F2
plants of cross Pusa Basmati 1121 X INGR15001 with SSR markers RM 141738 & RM 3484 for the presence of qBL3 and qBL7, 24 plants were found to be positive for qBL3, 5 plants for qBL7 and one plant positive for both of the QTLs. Selected BasILs containing desirable variation for yield and quality component traits and backcross progenies showing presence of neck blast resistant QTLs can be further used in breeding programme to improve yield potential of basmati rice.
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Navdeep Singh (2021). Molecular characterization of basmati introgression lines for spikelet per panicle QTL (qSPP2.1) AND transfer of neck blast resistance in basmati rice (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India.