Efficacy of Ty genes against begomovirus species causing tomato leaf curl disease in Punjab
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Date
2023
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Punjab Agricultural University
Abstract
Tomato leaf curl disease (ToLCD) transmitted by vector whitefly (Bemisia tabaci) is major constraint in tomato production in the Indian subcontinent. During 2021-22 symptoms noticed on leaf curl disease infected tomato were yellowing, mild to severe curling of leaves, mosaic, puckering, stunting, excessive flowering and rudimentary leaves. Symptom variability was more in rainy season compared to winter season. Infected plants showed the association of tomato leaf curl NewDelhi virus (ToLCNDV), tomato leaf curl Palampur virus (ToLCPalV) and tomato leaf curl Karnatka virus (ToLCKV) along with associated satellites by PCR assay. Partial genome characterization of begomovirus from infected samples confirmed the association of ToLCNDV, ToLPalV, ToLKV from these samples. Apart from these chilli leaf curl India virus (ChiLCV), tomato leaf curl Joydebpur virus (ToLCJoV), croton yellow mosaic virus (CYVMV) along with a new species of papaya leaf crumple virus were (PLCrV) were recorded for the first time from tomato in Punjab. Plant age 20 to 40 days after sowing was found to be most susceptible stage to begomovirus infection as considerable yield loss (up to 90.4 %) was observed during inoculation of predominant begomovirus. ToLCNDV along with beta satellites was found to cause maximum yield loss compared to helper virus alone. The kinetics of virus multiplication in tomato genotype with different Ty gene(s) combinations showed accumulation of viral genomic unit and symptom severity were lower in tomato genotypes (PVB-4-1, NLLR-1) carrying Ty-1+Ty-2+Ty-3 and genotype CA-4 carrying ty-5+Ty-6 gene combination as compared to susceptible genotype Punjab Chuhhara (1179.18 x106) when inoculated with ToLCNDV+beta satellite.
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Nayak, Lopamudra (2023). Efficacy of Ty genes against begomovirus species causing tomato leaf curl disease in Punjab (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.