DEVELOPMENT OF FUNCTIONAL MUTANTS, CLONING AND EXPRESSIONAL ANALYSIS OF RESISTANCE GENE ANALOGS (RGAs) AND TRANSCRIPTION FACTOR (TF) GENES IN FRENCH BEAN (Phaseolus vulgaris L.)
| dc.contributor.advisor | B., FAKRUDIN | |
| dc.contributor.author | B., DIVYA | |
| dc.contributor.other | H. B, LINGAIAH | |
| dc.contributor.other | T. S., AGHORA | |
| dc.contributor.other | V., DEVAPPA | |
| dc.contributor.other | C., ASHWAT | |
| dc.date.accessioned | 2020-11-18T05:20:05Z | |
| dc.date.available | 2020-11-18T05:20:05Z | |
| dc.date.issued | 2016-06 | |
| dc.description.abstract | The present study was undertaken to create spectral variability and to develop functional mutants using Ethyl Methyl Sulphonate (EMS) in the background of the cv. Arka Anoop of french bean. The EMS at 0.25% was found to be the LD50. A set of 1500 plants in M1 and similar population in plant-to-row manner in M2 generation was studied for spectral variability. A wide range of variability for both qualitative and quantitative traits including pole type, thin, thick, dark green and pale green colour and variegated leaved, pink flowered and curved pod mutants in varying frequencies was recorded. A set of ten mutant entries from each mutant category constituting 100 mutant lines was evaluated in M3 generation. Cloning and sequence analysis of 95 RGA clones revealed 85% - 99% similarity to known R genes and recorded P-Loop (13 clones), kinase (4 clones) and TIR (4 clones) in the NBS-LLR domains that fell into six distinct phylogenetic groups. The in silico genome wide identification of Transcription Factors (TFs) lead to the identification of 58 TF families with maximum homology in french bean. A set of 20 TF genes and 10 RGAs was tested for expression in manifesting the rust and the MYMV diseases. Nine RGAs and AP2-2, AP2-3, AP2-4, AP2-5, B3-1, B3-2, B3- 3, BHLH-1, BHLH-2, BHLH-3, BHLH-4, BHLH-5, BZIP-1, BZIP-2 and BZIP-3 TFs found upregulated in rust resistance. COHFBRGA-3, 4, 5 and 7, AP2-3, AP2-5, B3-2, B3-3, BHLH-4, BZIP-1 and BZIP-2 genes down-regulated and COHFBRGA-2, 25, 27 and 38 genes of french bean were up-regulated in MYMV resistance manifestation compared to control. | en_US |
| dc.identifier.other | UHS13PGD69 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810155160 | |
| dc.keywords | FRENCH BEAN, T298 | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 270 ( Due to pdf. conversion there is a difference in printed & soft copy ) | en_US |
| dc.publisher | University of Horticultural Sciences, Bagalkot (COLLEGE OF HORTICULTURE, GKVK POST, BANGALURU) | en_US |
| dc.research.problem | Thesis | en_US |
| dc.sub | Horticulture | en_US |
| dc.theme | DEVELOPMENT OF FUNCTIONAL MUTANTS, CLONING AND EXPRESSIONAL ANALYSIS OF RESISTANCE GENE ANALOGS (RGAs) AND TRANSCRIPTION FACTOR (TF) GENES IN FRENCH BEAN (Phaseolus vulgaris L.) | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | DEVELOPMENT OF FUNCTIONAL MUTANTS, CLONING AND EXPRESSIONAL ANALYSIS OF RESISTANCE GENE ANALOGS (RGAs) AND TRANSCRIPTION FACTOR (TF) GENES IN FRENCH BEAN (Phaseolus vulgaris L.) | en_US |
| dc.type | Thesis | en_US |