Effect of Zinc Nanoparticles on physiology of Mungbean [Vigna radiata (L.) Wilczek] under moisture stress condition
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Date
2020
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DRPCAU, Pusa
Abstract
The present study entitled ‗‗Effect of zinc oxide Nanoparticles on the physiology of Mungbean [Vigna radiata (L.) Wilczek] under moisture stress conditions‘‘. The experiment was performed in laboratory condition. Screening of 20 mungbean genotypes was done in order to identify the contrast set of moisture stress tolerant and susceptible genotype via evaluation of germination percentage and seedling traits (7-days old seedling). The experiment was performed in laboratory condition in Petri plate, under 1st objective to identify the optimum concentration of ZnO Nanoparticles in both moisture stress tolerant and susceptible genotypes based on germination percentage and seedling traits (7-days old seedlings) under normal as well as moisture stress condition. And further in IInd objective the effect of seed priming and foliar application with optimum concentration of ZnO Nanoparticles on morpho-physiological and biochemical parameters in 10 days old seedling were studied in both identified moisture stress tolerant (SPM-19-42) and susceptible genotype (SPM-19-53) under normal and moisture stress condition. The screening experiment is conducted with twenty Mungbean genotypes to classify the contrasting collection of Mungbean genotypes based on changes in physiological traits and germination related parameters. In screening experiment moisture stress was induced chemically with the help of PEG 6000 at different concentration (10 % and 20%). The selection carried out based on germination
percentage, seedling length, seedling dry weight, seedling vigour-I, and seedling vigour-II. Now both mungbean genotypes have been treated with different concentrations of zinc oxide Nanoparticles. The first objective was to define the optimum concentration of zinc oxide Nanoparticles based on germination percentage, germination capacity, root length, shoot length, seedling dry weight, seedling vigour-I, and seedling vigour-II. In the second objective, the response of pre-seed soaking treatment to an optimum concentration of ZnO Nanoparticles on mungbean seedling mechanism was studied in both control and moisture stress conditions. This was accomplished by observation of physiological parameters such as relative water content, membrane stability index and photosynthetic pigment (chlorophyll-a, chlorophyll-b, total chlorophyll, and Carotenoid content), SPAD value, chlorophyll stability index and lipid peroxidation. Morphological parameters such as plant height, leaf area, specific leaf area, specific leaf weight, dry matter portioning, and biochemical parameters such as peroxidase, superoxide dismutase and Proline were taken under consideration.
Overall moisture stress has adverse effects on seed germination, RWC, pigment content and also on dry matter partitioning in mungbean. Among the concentrations the pre-seed soaking with 50 ppm of ZnO Nanoparticles was effective in improvement of seedling growth and physiology of mungbean under normal as well as moisture stress conditions. The performance under seed priming along with foliar application with optimum concentration of ZnO Nanoparticles (50 ppm) was most effective among treatments in improving physiological and biochemical traits and overall plant growth of mungbean seedlings of both tolerant and susceptible genotypes under normal as well as water stress condition. Seed priming and foliar application with a concentration of 50 ppm of ZnO Nanoparticles can therefore be an effective alternative approach to reducing the adverse affect of moisture stress on mungbean seedling. However, more research is required under field conditions, to use ZnO NPs to reduce adverse affect of moisture stress in mungbean crop.