DEVELOPMENT OF Pasteurella multocida BIVALENT OUTER MEMBRANE PROTEIN BASED VACCINE ENTRAPPED IN ALUMINIUM HYDROXIDE NANOPARTICLES AND EVALUATION OF ITS IMMUNE RESPONSE IN MICE
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Date
2017-07
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Assam Agricultural University, Khanapara,Guwahati
Abstract
Swine pasteurellosis is one of the most economically important diseases of pig caused by Pasteurella multocida(P. multocida) capsular types A and D and these organisms are commensals and opportunistic pathogens in the upper respiratory tract in pig. The vaccine prepared from P52 stain of serotype B:2 of P. multocida is effective only in controlling bovine pasteurellosis but not swine pasteurellosis due to antigenic variation among capsular types A and D. In the present study, an attempt was made to develop a bivalent vaccine that could elicit immunity against both the capsular types of P.multocida.
Whole outer-membrane proteins (OMP) were extracted from P.multocida capsular types A and D, and were mixed together in the ratio of 1:1 forming bivalent outer-membrane proteins. The bivalent OMP was adsorbed onto aluminum hydroxide nanoparticles. The size of aluminum hydroxide nanoparticles adsorbed outer-membrane protein was found to be in the range of 120 to 130 nm.
The aluminum hydroxide nanoparticles adjuvanted bivalent OMP based vaccine has shown quickest immune kinetics in terms of IgG response as compared to aluminum hydroxide microparticlesadjuvanted bivalent bacterin vaccine against Pasteurella multocida capsular type A. However, the IgG response against P. multocida capsular type A stimulated by aluminum hydroxide nanoparticles adjuvanted bivalent OMP based vaccine could not last longer compared to aluminum hydroxide microparticle adjuvanted bivalent bacterin. The immune kinetics in terms of IgG response against P. multocida capsular type D stimulated by aluminum hydroxide nanoparticles adjuvanted OMP was found to be similar to aluminum hydroxide microparticlesadjuvanted bivalentbacterin vaccine. The aluminum hydroxide nanoparticles adjuvanted bivalent OMP was efficient in stimulating IgG response in initial level against P.multocida capsular type A that may be helpful in use of vaccine formulation during the outbreak of the disease.
Finally, the local inflammation induced by aluminum hydroxide nanoparticles in the injection sites was found to be milder than that induced by aluminum hydroxide microparticles.
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