DEVELOPMENT OF MONOSPECIFIC ANTI-BEEF SERA
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Date
1995
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES Mannuthy - Thrissur
Abstract
Agar gel immunodiffusion is a simple and reliable test
for identifying the species origin of meat, povided the
antisera to be used are monospecif ic. A study was
undertaken to make Rabbit anti-cattle serum (RAGS) and
Rabbit anti-buffalo serum (RABS) monospecific by absorption
with the freeze dried sera of goat (GFD), buffalo (BFD),
cattle (GFD) and a combination of GFD and GFD or, GFD and
BFD. Though it was found that the RAGS was made monospecific
by absorption with BFD, production of monospp- ;ific
RABS through absorption with GFD or GFD, is more desirable.
Absorption of RABS with GFD alone enabled to identify both
beef and buffalo meat samples which can be further
confirmed by RABS absorbed with GFD. RAGS absorbed with
BFD and RABS absorbed with GFD could identify a level of
25 per cent or above adulteration with beef and buffalo
beef respectively.
Filter paper was found to be good carrier of beef and
buffalo meat extract antigens and storing it for upto 30
days did not influence the test result with unabsorbed
antisera. All the three eluants, NaCl, PBS and PBS-T were
found to be equally useful for elution of the meat antigen
from the dried filter paper.
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