EVALUATION OF COLD AND HOT EXTRACTS OF ARGYREIA NERVOSA AND SWELLNIL - AN AYURVEDIC OINTMENT FOR WOUND HEALING PROPERTIES IN ANIMALS - EXPERIMENTAL, HISTOLOGICAL, BIOMECHANICAL AND CLINICAL STUDIES
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Date
2002
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AAU, Anand
Abstract
Each of 12 healthy male buffalo calves were subjected to eight excisional wounds, four on each side of thoracolumbar region to evaluate the wound healing properties of Argyreia nervosa leaves and an ayurvedic ointment Swellnil . The wound healing was evaluated clinically and by histological and biomechanical examination of excised healing tissues collected at seven, 14 and 28 days. Almost all wounds under different treatments, showed inflammation upto third day. Pink-reddish coloured granulations below the scab fourth day onwards, clearly evident contraction from 14th day onwards, progressive epithelialization in behveen 14th to 21st days and complete healing between 21st to 28th day were observed in wounds under different treatments. Good angiogenic activity under hot extract ointment at seven day stage of regeneration, better development of all the layers of epidermis and dermis under cold and hot extract ointments at 14th day of regeneration, development of elastic fibres and bundles of collagen fibres similar to normal skin at 28th day of regeneration, intense alkaline phosphatase at early stage of regeneration which gradually decreased at later stage, more cellular and less fibrous dermis which changed to more fibrous with less cellularity at later stage of healing were observed. The tensile strength and extensibility of the tissue increased gradually from seven to 28th day of regeneration with no significant difference between the treatment was observed. In clinical trials A. nervosa dry leaves husk showed antiinflammatory properties with absorption of exudate and early establishment of granulation tissue, while cold and hot alcoholic extracts ointments and Swellnil helped in normalization of the wound condition. The husk acts as a scaffold and traps the exudate which provides warmth to the wound, thus providing ideal environment for granulation tissue to form. Cold and hot extracts of A. nervosa leaves showed marked antifungal activity against the fungi isolated from the clinical cases of wounds. The bacterial growth was noticed throughout the study and in vitro antibacterial activity of the cold and hot alcoholic extracts of A. nervosa leaves could not be established. From the present study it can be concluded that the cold and hot alcoholic extracts ointments of A. nervosa leaves, dry leaves husk of A. nervosa and Swellnil ointments have no deleterious effect on wound healing. In clinical cases they help in normalization of the healing process. Further, early angiogenic property of hot extract ointment establishes its superiority over other ointments used and dry husk of A. nervosa has intrinsic wound healing capabilities.
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Keywords
VETERINARY SURGERY, EVALUATION