Isolation and characterization of non-rhizobial endophytic Plant Growth Promoting Bacteria (PGPB) from root nodules of leguminous crops and their efficacy in groundnut (Arachis hypogaea L.)
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Date
2014
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Publisher
AAU, Anand
Abstract
Present investigation was carried out to isolate efficient non
rhizobial root nodule endophytic PGPB from leguminous crops viz. Glycine max
(soybean), Vigna radiata (mung bean), Vigna unguiculata (cow pea), Arachis
hypogaea (groundnut) and Vigna mungo (black gram) from AAU, Anand, Gujarat.
Out of thirty four, fourteen bacterial isolates were able to grow and flourish on
nitrogen free medium. These fourteen isolates were further subjected to
amplification of nifH gene and seven were found to be amplifying nifH gene of
size ~380 bp which by 16S rRNA sequencing characterized as Enterobacter cloacae
AS1 (Accs no. KF758544), Chryseobacterium indologenes AM2 (Accs no. KF758545),
Enterobacter cloacae ACP3 (Accs no. KF758546), Klebsiella pneumoniae AG4 (Accs
no. KF758547), Pseudomonas aeruginosa ABG5 (Accs no. KF758548), Enterobacter
ludwigii ABG6 (Accs no. KF758549) and Klebsiella variicola ABG7 (Accs no.
KF758550).
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Keywords
agriculture, microbiology, method