CONTROL ON Mycogone perniciosa (Magnus) Delacr. CAUSING WET BUBBLE DISEASE OF Agaricus bisporus (Lang) Sing.
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Date
2013
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Punjab Agricultural University
Abstract
Present investigation entitled “control on Mycogone perniciosa (Magnus) Delacr. causing wet bubble disease of Agaricus bisporus (Lang) Sing.” was conducted for in vitro toxicity analysis of four chemicals namely, carbendazim, formaldehyde, thiram and zineb against Agaricus bisporus strain (U3). A. bisporus was found sensitive to formaldehyde @ 10ppm. Carbendazim , thiram and zineb were less inhibitory for the growth of Agaricus bisporus. These chemicals further used against Mycogone perniciosa to show complete suppression (100%) of M. perniciosa was at 10 ppm carbendazim and 30 ppm formaldehyde whereas only 26.15 and 21.54% inhibition were observed in case of zineb and thiram upto 40ppm concentration. Carbendazim was selected for the in vivo control of M. perniciosa @5 and 10 times higher concentrations than that of promising in vitro dose (10ppm). Carbendazim (50ppm or 100ppm) was added at the time of casing and spawning + casing time. The maximum disease incidence (45.5%) of M. perniciosa was recorded in negative control with 3.8kg/100kg yield loss and minimum yield loss (1.8kg/100kg) with 100ppm carbendazim when sprayed at the time of spawning + casing. High yield potential of healthy fruit bodies was recorded in positive control (11.6kg/100kgcompost) as well as 100ppm carbendazim sprayed at casing (11.9kg/100kgcompost) in uninoculated M. perniciosa bags. Out of bags inoculated with Mycogone perniciosa , maximum yield of healthy mushroom was observed when100ppm carbendazim sprayed at spawning and casing (10kg/100kg compost) as compare to negative control (4.56kg/100kg).The residue analysis of mushrooms appeared in 100 ppm carbendazim sprayed at time spawning and casing was found below the detectable limit to suggest safe consumption of mushrooms
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Keywords
Microbiology, Agaricus bisporus, Biochemistry, chemicals